TPDB

3D PDB MODEL

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TPDB ID TPDB61407

Peptide_Name

FUSO

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

C-terminal: Amidation N-terminal: Acetylation

Sequence

Ac-CGLFEALLELLESLWELLLEA-NH2

FASTA

>TPDB61407|Cell-penetrating Peptides|length=21
Ac-CGLFEALLELLESLWELLLEA-NH2

Formula C113H178N22O33S1

Absent Amino Acids RNDQHIKMPTYV

Common Amino Acids L

Mass 2404.84

PI 2.90

Basic Residues 0

Acidic Residues 5

Hydrophobic Residues 13

Net Charge -5

Boman Index 1.64

Hydrophobicity 1.119

Aliphatic Index 176.67

Half Life

Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 228.71

Polar Residues 2

number

2

A

0

R

0

N

0

D

1

C

5

E

0

Q

1

G

0

H

0

I

9

L

0

K

0

M

1

F

0

P

1

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 2 R: 0 N: 0 D: 0 C: 1 E: 5 Q: 0 G: 1 H: 0 I: 0 L: 9 K: 0 M: 0 F: 1 P: 0 S: 1 T: 0 W: 1 Y: 0 V: 0

Length

21

Formula

C113H178N22O33S1

Mass

2404.84

PI

2.90

Net Charge

-5

Boman Index

1.64

Hydrophobicity

1.119

C_terminal_Modification

Amidation

Chirality

L

Ionic_State

Anionic

Linearity

Linear

N_terminal_Modification

Acetylation

SMILES

N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Human Embryonic Kidney-Derived Cells 293Tt , 3T6 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Anionic

Source of Peptide Protein derived

Cargo Type VLPs*(MPyV virus-like particles labeled with Alexa Fluor 488)

Uptake Efficiency Significantly lower relative MFI was detected than control VLPs*

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSCTTC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Human Embryonic Kidney-Derived Cells 293Tt , 3T6 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Anionic

Source of Peptide Protein derived

Cargo Type PsVs of MPyV(SV40 and MusPV)

Uptake Efficiency

None of the three types of PsVs was enhanced by the FUSO peptide, except for a slight enhancement of MusPV transduction at 5 μM.

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSCTTC

DSSP_Secondary_Structure

CCCSSSCTTC