TPDB

3D PDB MODEL

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TPDB ID TPDB61287

Peptide_Name

CPAb (Tetra-arginine motif in the VH CDR3 loop)

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 5-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

Chemical: TAMRA-labeled

Sequence

ARRRR

FASTA

>TPDB61287|Cell-penetrating Peptides|length=5
ARRRR

Formula C27H55N17O6

Absent Amino Acids NDCEQGHILKMFPSTWYV

Common Amino Acids R

Mass 713.84

PI 12.88

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 1

Net Charge +4

Boman Index 3.07

Hydrophobicity -3.240

Aliphatic Index 20.00

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

1

A

4

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

0

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 1 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

5

Formula

C27H55N17O6

Mass

713.84

PI

12.88

Net Charge

+4

Boman Index

3.07

Hydrophobicity

-3.240

C_terminal_Modification

Free

Chemical_Modification

TAMRA-labeled

Chirality

L

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line A-431, HeLa, And Skbr-3 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Non-natural residues Linear L

Source of Peptide Synthetic

Cargo Type eGFP

Subcellular Localization Cytosol

Uptake Efficiency

CPAb-TAMRA resulted in a distinctive homogeneous distribution of TAMRA fluorescence in the HeLa cells. A diffuse fluorescence was observed in cells treated with CPAb-eGFP.

Uptake Mechanism Receptor mediated endocytosis

In Vitro or In Vivo In vitro

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line A-431, HeLa, And Skbr-3 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Truncated Pseudomonas aeruginosa exotoxin.

Subcellular Localization Cytosol

Uptake Mechanism Receptor mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCC

DSSP_Secondary_Structure

CCCCC