TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB47648

Peptide_Name

Rapakinin - ACE inhibitor Rapakinin – hypotensive peptide

Activity

ACE inhibitors Antihypertensive

Activity_category_statistics

Cardiometabolic peptides

Database_Source

Peptipedia AHTPDB AHTpin

Sequence_Type

standard

Has_Modification No

Description A 3-aa standard natural multi-activity (ACE inhibitors and Antihypertensive) peptide sequence curated from Peptipedia, AHTPDB, and AHTpin, with an available 3D structural model.

Sequence

RIY

FASTA

>TPDB47648|ACE inhibitors;Antihypertensive|length=3
RIY

Formula C21H34N6O5

Absent Amino Acids ANDCEQGHLKMFPSTWV

Common Amino Acids RIY

Mass 450.54

PI 9.88

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 2

Net Charge +1

Boman Index -0.44

Hydrophobicity -0.433

Aliphatic Index 130.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 330.72

Polar Residues 1

number

0

A

1

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

0

L

0

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

3

Formula

C21H34N6O5

Mass

450.54

PI

9.88

Net Charge

+1

Boman Index

-0.44

Hydrophobicity

-0.433

Chemical_Mass

450.5305

Monoisotopic_Mass

450.2583

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity ACE inhibitors

Activity Measurement 28.00 µM

Function Inhibitor of Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) (MEROPS ID: M02-001)

Detail Activity ACE inhibitor

Activity Code ah

Source & Reference

Database Source NA

Authors Marczak E. D., Usui H., Fujita H., Yang Y., Yokoo M., Lipkowski A. W., Yoshikawa M.

Title New antihypertensive peptides isolated from rapeseed. Peptides, 24, 791-798, 2006

Year 2006

Literature Source Journal

Other

Number of Residues 3

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@H](CCCNC(=N)N)C(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O InChI=1S/C21H34N6O5/c1-3-12(2)17(27-18(29)15(22)5-4-10-25-21(23)24)19(30)26-16(20(31)32)11-13-6-8-14(28)9-7-13/h6-9,12,15-17,28H,3-5,10-11,22H2,1-2H3,(H,26,30)(H,27,29)(H,31,32)(H4,23,24,25)/t12-,15-,16-,17-/m0/s1 InChIKey=FNXCAFKDGBROCU-STECZYCISA-N Appetite regulating (anorexigenic) peptide according to the BIOPEP-UWM database of bioactive peptides (ID 10135) Hypotensive peptide according to the BIOPEP-UWM database of bioactive peptides (ID 10134) Opioid antagonist according to the BIOPEP-UWM database of bioactive peptides (ID 10133)

Evidence 2 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Antihypertensive

Activity Measurement 28.00 µM

Function Inhibitor of Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) (MEROPS ID: M02-001)

Detail Activity ACE inhibitor

Activity Code ah

Source & Reference

Database Source AHTPDB

Authors Marczak E. D., Usui H., Fujita H., Yang Y., Yokoo M., Lipkowski A. W., Yoshikawa M.

Title New antihypertensive peptides isolated from rapeseed. Peptides, 24, 791-798, 2006

Year 2006

Literature Source Journal

Other

Source Activity Label	Source Definition
Antihypertensive	AHTPDB manually curated experimentally validated antihypertensive peptide annotation.

Number of Residues 3

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@H](CCCNC(=N)N)C(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O InChI=1S/C21H34N6O5/c1-3-12(2)17(27-18(29)15(22)5-4-10-25-21(23)24)19(30)26-16(20(31)32)11-13-6-8-14(28)9-7-13/h6-9,12,15-17,28H,3-5,10-11,22H2,1-2H3,(H,26,30)(H,27,29)(H,31,32)(H4,23,24,25)/t12-,15-,16-,17-/m0/s1 InChIKey=FNXCAFKDGBROCU-STECZYCISA-N Appetite regulating (anorexigenic) peptide according to the BIOPEP-UWM database of bioactive peptides (ID 10135) Hypotensive peptide according to the BIOPEP-UWM database of bioactive peptides (ID 10134) Opioid antagonist according to the BIOPEP-UWM database of bioactive peptides (ID 10133)

Evidence 3 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Antihypertensive

Activity Measurement 0.00 µM

Function Peptide dilating mesenteric artery in rats

Detail Activity hypotensive

Activity Code hyp

Source & Reference

Database Source AHTPDB

Authors Yamada Y., Iwasaki M., Usui H., Ohinata K., Marczak E. D., Lipkowski A. W., Yoshikawa M.

Title

Rapakinin, an anti-hypertensive peptide derived from rapeseed protein, dilates mesenteric artery of spontaneously hypertensive rats via the prostaglandin IP receptor followed by CCK1 receptor. Peptides, 31, 909–914, 2010

Year 2010

Literature Source Journal

Other

Number of Residues 3

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@H](CCCNC(=N)N)C(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O InChI=1S/C21H34N6O5/c1-3-12(2)17(27-18(29)15(22)5-4-10-25-21(23)24)19(30)26-16(20(31)32)11-13-6-8-14(28)9-7-13/h6-9,12,15-17,28H,3-5,10-11,22H2,1-2H3,(H,26,30)(H,27,29)(H,31,32)(H4,23,24,25)/t12-,15-,16-,17-/m0/s1 InChIKey=FNXCAFKDGBROCU-STECZYCISA-N Appetite regulating (anorexigenic) peptide according to the BIOPEP-UWM database of bioactive peptides (ID 10135 Opioid antagonist according to the BIOPEP-UWM database of bioactive peptides (ID 10133) Inhibitor of Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) (MEROPS ID: M02-001) according to the AHTPDB database the BIOPEP-UWM database of bioactive peptides (ID 7821) the EROP-Moscow database

Number_of_Residues

3