TPDB

3D PDB MODEL

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TPDB ID TPDB41559

Peptide_Name

AEM-28

Activity

Anticancer

Activity_category_statistics

Cancer-related peptides

Database_Source

CancerPPD 2.0

Sequence_Type

standard

Has_Modification No

Description A 28-aa standard natural anticancer peptide sequence curated from CancerPPD 2.0, with an available 3D structural model.

Sequence

LRKLRKRLLRDWLKAFYDKVAEKLKEAF

FASTA

>TPDB41559|Anticancer|length=28
LRKLRKRLLRDWLKAFYDKVAEKLKEAF

Formula C166H273N47O38

Absent Amino Acids NCQGHIMPST

Common Amino Acids LK

Mass 3535.29

PI 10.84

Basic Residues 10

Acidic Residues 4

Hydrophobic Residues 14

Net Charge +6

Boman Index 6.83

Hydrophobicity -0.700

Aliphatic Index 104.64

Half Life

Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 6990

Absorbance 280nm 197.72

Polar Residues 1

number

3

A

4

R

0

N

2

D

0

C

2

E

0

Q

0

G

0

H

0

I

6

L

6

K

0

M

2

F

0

P

0

S

0

T

1

W

1

Y

1

V

Amino Acid Distribution

A: 3 R: 4 N: 0 D: 2 C: 0 E: 2 Q: 0 G: 0 H: 0 I: 0 L: 6 K: 6 M: 0 F: 2 P: 0 S: 0 T: 0 W: 1 Y: 1 V: 1

Length

28

Formula

C166H273N47O38

Mass

3535.29

PI

10.84

Net Charge

+6

Boman Index

6.83

Hydrophobicity

-0.700

C_terminal_Modification

Free

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Anti-inflammatory and Anticancer

Origin

Synthetic

Evidence 1 Activity

Activity

Activity Raw

~20% cell viability at 10 µg/mL

Detail Source Activity Anticancer

Target

Cancer Type Colorectal Cancer

Cell Line CT-26

Tissue Affected Colon

Assay & Model

Assay MTS assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Raw

~60% cell viability at 10 µg/mL

Detail Source Activity Anticancer

Target

Cancer Type Colon Cancer

Cell Line ID8

Tissue Affected Colon

Assay & Model

Assay MTS assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Evidence 3 Activity

Activity

Activity Raw

~60% cell viability at 10 µg/mL

Detail Source Activity Anticancer

Target

Cancer Type Ovarian Cancer

Cell Line OV-2008

Tissue Affected Ovary

Assay & Model

Assay MTS assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Evidence 4 Activity

Activity

Activity Raw

~50% cell viability at 10 µg/mL

Detail Source Activity Anticancer

Target

Cancer Type Ovarian Cancer

Cell Line SK-OV-3

Tissue Affected Ovary

Assay & Model

Assay MTS assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Evidence 5 Activity

Activity

Activity Raw

~70% cell viability at 10 µg/mL

Detail Source Activity Anticancer

Target

Cancer Type Colon Cancer

Cell Line CaCo-2

Tissue Affected Colon

Assay & Model

Assay MTS assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020