TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB41096

Peptide_Name

Pal-pFL-N-Ter-TAT

Activity

Anticancer

Activity_category_statistics

Cancer-related peptides

Database_Source

CancerPPD 2.0 dbACP

Sequence_Type

standard

Has_Modification No

Description A 32-aa standard natural anticancer peptide sequence curated from CancerPPD 2.0 and dbACP, with an available 3D structural model.

Modification

C-terminal: TAT Chemical: Dansylglycine = fluorescent amino acid residue located in the side chain of lysine N-terminal: Palmitic acid N-terminal: pFL - a hydrophobic peptide fragment (FPWWWPFL) rich in tryptophan (W)

Sequence

FPWWWPFLRDVFTKGYGFGLGRKKRRQRRRPQ

FASTA

>TPDB41096|Anticancer|length=32
FPWWWPFLRDVFTKGYGFGLGRKKRRQRRRPQ

Formula C196H289N61O39

Absent Amino Acids ANCEHIMS

Common Amino Acids R

Mass 4123.84

PI 12.53

Basic Residues 10

Acidic Residues 1

Hydrophobic Residues 11

Net Charge +9

Boman Index 0.50

Hydrophobicity -1.306

Aliphatic Index 33.44

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 17990

Absorbance 280nm 436.24

Polar Residues 4

number

0

A

7

R

0

N

1

D

0

C

0

E

2

Q

4

G

0

H

0

I

2

L

3

K

0

M

4

F

3

P

0

S

1

T

3

W

1

Y

1

V

Amino Acid Distribution

A: 0 R: 7 N: 0 D: 1 C: 0 E: 0 Q: 2 G: 4 H: 0 I: 0 L: 2 K: 3 M: 0 F: 4 P: 3 S: 0 T: 1 W: 3 Y: 1 V: 1

Length

32

Formula

C196H289N61O39

Mass

4123.84

PI

12.53

Net Charge

+9

Boman Index

0.50

Hydrophobicity

-1.306

C_terminal_Modification

TAT

Chemical_Modification

Dansylglycine = fluorescent amino acid residue located in the side chain of lysine

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Palmitic acid pFL - a hydrophobic peptide fragment (FPWWWPFL) rich in tryptophan (W)

Nature

Anticancer

Origin

Analogue of N-terminal fragment of the VDAC1 protein

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 5.5 ± 1.1 µM

Detail Source Activity Anticancer

Activity Measurement 5.5

Target

Cancer Type Skin Cancer

Cell Line A-375

Tissue Affected Skin

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 31.17 µM

Detail Source Activity Anticancer

Activity Measurement 31.17 µM

Target

Cancer Type Lung Cancer

Cell Line A-549

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 11.85 µM

Detail Source Activity Anticancer

Activity Measurement 11.85 µM

Target

Cancer Type Breast Cancer

Cell Line MCF-7

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2021