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Peptide record

TPDB40470

Neurotoxin Toxicity standard
35 amino acids
Basic Information
3D PDB MODEL
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TPDB40470
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 ToxinPred 3.0
standard
No
A 35-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and ToxinPred 3.0, with an available 3D structural model.
Sequence
DDCLGMFSSCDPNNDKCCPNRKCSRKDQWCKYQLW
Physicochemical Analysis
C174H264N52O55S7
AEHITV
C
4188.75
7.62
6
5
7
+1
6.89
-1.183
22.29
Mammalian: 1.1 hour Yeast: 3 min E.coli: >10 hour
12865
307.13
15
Residue Composition
number
0
A
2
R
3
N
5
D
6
C
0
E
2
Q
1
G
0
H
0
I
2
L
4
K
1
M
1
F
2
P
3
S
0
T
2
W
1
Y
0
V
Amino Acid Distribution
A: 0 R: 2 N: 3 D: 5 C: 6 E: 0 Q: 2 G: 1 H: 0 I: 0 L: 2 K: 4 M: 1 F: 1 P: 2 S: 3 T: 0 W: 2 Y: 1 V: 0
Chemical Descriptors
35
C174H264N52O55S7
4188.75
7.62
+1
6.89
-1.183
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Gating-modifier toxin that inhibits both sodium (Nav) and calcium (Cav3) channels by inducing hyperpolarizing shift in voltage-dependence of activation and steady state inactivation. Inhibits Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.6/SCN6A, Nav1.7/SCN9A and Cav3.1/CACNA1G sodium and calcium channels at nanomolar concentrations (IC(50)=81-301 nM). Surprisingly, selectively slows fast inactivation of Nav1.3/SCN3A. Also shows moderate inhibition of Cav3.2/CACNA1H calcium channels (IC(50)=1233 nM). Ex vivo, nearly ablates neuronal mechanosensitivity in afferent fibers innervating the colon and the bladder. In vivo, in a mouse model of irritable bowel syndrome, intracolonic administration of the toxin reverses colonic mechanical hypersensitivity. {ECO:0000269|PubMed:32826759}.

Target

voltage-gated sodium channel (Nav) voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Theraphosa apophysis (Goliath pinkfoot tarantula) (Pseudotheraphosa apophysis)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Gating-modifier toxin that inhibits both sodium (Nav) and calcium (Cav3) channels by inducing hyperpolarizing shift in voltage-dependence of activation and steady state inactivation. Inhibits Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.6/SCN6A, Nav1.7/SCN9A and Cav3.1/CACNA1G sodium and calcium channels at nanomolar concentrations (IC(50)=81-301 nM).
Mu/omega-theraphotoxin-Tap1a (Mu/omega-TRTX-Tap1a)
spider
Neurotoxin 10 (Hwtx-1) family, 59 (Tltx) subfamily
Evidence at protein level
Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:32826759}.
Additional Detail Fields 7 fields
Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 10 (Hwtx-1) family, 59 (Tltx) subfamily
Mu/omega-theraphotoxin-Tap1a (Mu/omega-TRTX-Tap1a)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:32826759}.
spider