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Peptide record

TPDB40382

Neurotoxin Toxicity standard
33 amino acids
Basic Information
3D PDB MODEL
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TPDB40382
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 ToxinPred 3.0
standard
No
A 33-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and ToxinPred 3.0, with an available 3D structural model.
Sequence
ADCLAHLKLCKKNNDCCSKKCSRRGTNPEQRCR
Physicochemical Analysis
C149H260N56O47S6
IMFWYV
C
3780.41
9.30
10
3
5
+6
12.87
-1.197
41.52
Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour
375
9.92
13
Residue Composition
number
2
A
4
R
3
N
2
D
6
C
1
E
1
Q
1
G
1
H
0
I
3
L
5
K
0
M
0
F
1
P
2
S
1
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 2 R: 4 N: 3 D: 2 C: 6 E: 1 Q: 1 G: 1 H: 1 I: 0 L: 3 K: 5 M: 0 F: 0 P: 1 S: 2 T: 1 W: 0 Y: 0 V: 0
Chemical Descriptors
33
C149H260N56O47S6
3780.41
9.30
+6
12.87
-1.197
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: This toxin stabilizes ryanodine receptor 1 (RyR1) opening in a long-lasting subconductance state (60% of the full conductance state) (PubMed:27114612). Furthermore, it triggers calcium release from sarcoplasmic vesicles (31 nM are enough to induce a sharp release, and 65% of the total calcium is released after toxin (100 nM) addition) probably by acting as a cell-penetrating peptide (CPP) (PubMed:27114612). In addition, it has been shown to dose-dependently stimulate ryanodine binding to RyR1 (EC(50)=3.7 nM) (PubMed:27114612). It also augments the bell-shaped calcium-[3H]ryanodine binding curve that is maximal at about 10 uM calcium concentration (PubMed:27114612). It binds a different site as ryanodine (By similarity). It acts synergistically with caffeine (By similarity). In vivo, intracerebroventricular injection into mice induces neurotoxic symptoms, followed by death (By similarity). {ECO:0000250|UniProtKB:A0A1L4BJ42, ECO:0000250|UniProtKB:B8QG00, ECO:0000250|UniProtKB:P59868, ECO:0000250|UniProtKB:P60254, ECO:0000269|PubMed:27114612}.

Target

voltage-gated calcium channel (Cav) ryanodine receptor

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Vaejovis mexicanus (Mexican scorpion)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Ryanodine-sensitive calcium-release channel impairing toxin
This toxin stabilizes ryanodine receptor 1 (RyR1) opening in a long-lasting subconductance state (60% of the full conductance state) (PubMed:27114612). Furthermore, it triggers calcium release from sarcoplasmic vesicles (31 nM are enough to induce a sharp release, and 65% of the total calcium is released after toxin (100 nM) addition) probably by acting as a cell-penetrating peptide (CPP) (PubMed:27114612).
Vejocalcin (VjCa)
scorpion
Scorpion calcin family
Evidence at protein level
Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Ryanodine-sensitive calcium-release channel impairing toxin Secreted Toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:27114612}.
Additional Detail Fields 7 fields
Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Ryanodine-sensitive calcium-release channel impairing toxin Secreted Toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Ryanodine-sensitive calcium-release channel impairing toxin
Evidence at protein level
Scorpion calcin family
Vejocalcin (VjCa)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:27114612}.
scorpion