TPDB

3D PDB MODEL

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TPDB ID TPDB38625

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 23-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, and ToxinPred 3.0, with an available 3D structural model.

Sequence

TWEECCKNPGCRNNHVDRCRGQV

FASTA

>TPDB38625|Neurotoxin;Toxicity|length=23
TWEECCKNPGCRNNHVDRCRGQV

Formula C107H170N40O35S4

Absent Amino Acids AILMFSY

Common Amino Acids C

Mass 2705.01

PI 7.85

Basic Residues 5

Acidic Residues 3

Hydrophobic Residues 3

Net Charge +1

Boman Index 9.43

Hydrophobicity -1.335

Aliphatic Index 25.22

Half Life

Mammalian: 7.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5750

Absorbance 280nm 212.57

Polar Residues 9

number

0

A

3

R

3

N

1

D

4

C

2

E

1

Q

2

G

1

H

0

I

0

L

1

K

0

M

0

F

1

P

0

S

1

T

1

W

0

Y

2

V

Amino Acid Distribution

A: 0 R: 3 N: 3 D: 1 C: 4 E: 2 Q: 1 G: 2 H: 1 I: 0 L: 0 K: 1 M: 0 F: 0 P: 1 S: 0 T: 1 W: 1 Y: 0 V: 2

Length

23

Formula

C107H170N40O35S4

Mass

2705.01

PI

7.85

Net Charge

+1

Boman Index

9.43

Hydrophobicity

-1.335

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (PubMed:23533449). Is a specific blocker of the alpha-3-beta-4/CHRNA3-CHRNB4 image nAChR and may also block alpha-3-beta-4-alpha-5 (CHRNA3-CHRNB4-CHRNA5) channels (PubMed:23533449). Has possibly a distinct nAChR binding mode from other alpha-conotoxins, due to a different three residue motif (lacks the Ser-Xaa-Pro motif) (By similarity). In vivo, causes hyperactivity and behavioral disorders in mice following intracranial injection (PubMed:23533449). {ECO:0000250|UniProtKB:Q2I2R8, ECO:0000269|PubMed:23533449}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus regius (Crown cone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Mechanism

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (PubMed:23533449). Is a specific blocker of the alpha-3-beta-4/CHRNA3-CHRNB4 image nAChR and may also block alpha-3-beta-4-alpha-5 (CHRNA3-CHRNB4-CHRNA5) channels (PubMed:23533449).

Protein Name Alpha-conotoxin-like RgIB (Alpha-RGIB)

Venom Taxon Group cone snail

Protein Family Conotoxin A superfamily

Protein Existence Evidence at protein level

Keywords Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by venom duct. {ECO:0000305|PubMed:23533449}.

Keywords

Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin A superfamily

Protein_Name

Alpha-conotoxin-like RgIB (Alpha-RGIB)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by venom duct. {ECO:0000305|PubMed:23533449}.

Venom_Taxon_Group

cone snail