TPDB

3D PDB MODEL

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TPDB ID TPDB37541

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 32-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, and ToxinPred 3.0, with an available 3D structural model.

Sequence

RDCQEKWEYCIVPILGFVYCCPGLICGPFVCV

FASTA

>TPDB37541|Neurotoxin;Toxicity|length=32
RDCQEKWEYCIVPILGFVYCCPGLICGPFVCV

Formula C167H250N38O42S6

Absent Amino Acids ANHMST

Common Amino Acids C

Mass 3654.41

PI 4.44

Basic Residues 2

Acidic Residues 3

Hydrophobic Residues 14

Net Charge -1

Boman Index -0.17

Hydrophobicity 0.831

Aliphatic Index 97.19

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 8855

Absorbance 280nm 242.31

Polar Residues 9

number

0

A

1

R

0

N

1

D

6

C

2

E

1

Q

3

G

0

H

3

I

2

L

1

K

0

M

2

F

3

P

0

S

0

T

1

W

2

Y

4

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 1 C: 6 E: 2 Q: 1 G: 3 H: 0 I: 3 L: 2 K: 1 M: 0 F: 2 P: 3 S: 0 T: 0 W: 1 Y: 2 V: 4

Length

32

Formula

C167H250N38O42S6

Mass

3654.41

PI

4.44

Net Charge

-1

Boman Index

-0.17

Hydrophobicity

0.831

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: MuO-conotoxins are gating-modifier toxins that inhibit sodium current by trapping the domain II voltage sensor in the closed position to prevent opening of the sodium channel. This toxin shows high activity on Nav1.4/SCN4A (IC(50)=81 nM) and Nav1.8/SCN10A (IC(50)=96-529 nM). It also shows low activity on other sodium channels (Nav1.5/SCN5A, IC(50)=431-3900 nM > Nav1.1/SCN1A, IC(50)=3.3 uM Nav1.2/SCN2A, IC(50)=5.1-6.3 uM Nav1.3/SCN3A, IC(50)=2.2 uM Nav1.6/SCN8A, IC(50)=1.2-4.6 uM Nav1.7/SCN9A, IC(50)=2.3-5.5 uM) (PubMed:22609441). On Nav1.8/SCN10A, it causes a small left shift in the voltage dependence of activation and the voltage dependence of inactivation (PubMed:27026701). In addition, it has been shown to interact with lipid membranes (PubMed:27026701). {ECO:0000269|PubMed:22609441, ECO:0000269|PubMed:27026701}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus magnificus (Magnificent cone) (Darioconus magnificus)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

MuO-conotoxins are gating-modifier toxins that inhibit sodium current by trapping the domain II voltage sensor in the closed position to prevent opening of the sodium channel. This toxin shows high activity on Nav1.4/SCN4A (IC(50)=81 nM) and Nav1.8/SCN10A (IC(50)=96-529 nM).

Protein Name MuO-conotoxin MfVIA

Venom Taxon Group cone snail

Protein Existence Evidence at protein level

Keywords 3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:22609441}.

Keywords

3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Name

MuO-conotoxin MfVIA

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:22609441}.

Venom_Taxon_Group

cone snail