TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB35755

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 83-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

MWFKIQVLVLAITLITLGIQAEPNSSPNNPLIVEEDRAECAAVYERCGKGYKRCCEERPCKCNIVMDNCTCKKFISELFGFGK

FASTA

>TPDB35755|Neurotoxin;Toxicity|length=83
MWFKIQVLVLAITLITLGIQAEPNSSPNNPLIVEEDRAECAAVYERCGKGYKRCCEERPCKCNIVMDNCTCKKFISELFGFGK

Formula C412H660N110O119S10

Absent Amino Acids H

Common Amino Acids CE

Mass 9379.04

PI 7.48

Basic Residues 11

Acidic Residues 10

Hydrophobic Residues 32

Net Charge +1

Boman Index 18.05

Hydrophobicity -0.023

Aliphatic Index 84.58

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 8980

Absorbance 280nm 95.75

Polar Residues 23

number

5

A

4

R

5

N

2

D

8

C

8

E

2

Q

5

G

0

H

7

I

6

L

7

K

2

M

4

F

4

P

3

S

3

T

1

W

2

Y

5

V

Amino Acid Distribution

A: 5 R: 4 N: 5 D: 2 C: 8 E: 8 Q: 2 G: 5 H: 0 I: 7 L: 6 K: 7 M: 2 F: 4 P: 4 S: 3 T: 3 W: 1 Y: 2 V: 5

Length

83

Formula

C412H660N110O119S10

Mass

9379.04

PI

7.48

Net Charge

+1

Boman Index

18.05

Hydrophobicity

-0.023

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Antagonist of L-type calcium channels (Cav1/CACNA1) (PubMed:10098851). In GH3 neuroendocrinal cell line, it reversibly inhibits the A-type potassium current but does not block other potassium currents or calcium channels (PubMed:10098851). Shows an important acetylcholine-mediated antiarrhythmogenic effect in isolated hearts (PubMed:21115025). In vivo, causes paralysis in the posterior limbs and gradual decreases in movement and aggression during 24 hours at dose levels of 5 ug per mouse (PubMed:10098851). {ECO:0000269|PubMed:10098851, ECO:0000269|PubMed:21115025}.

Target

Target or Channel voltage-gated calcium channel (Cav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer)

Neurotoxin Type Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Mechanism

Antagonist of L-type calcium channels (Cav1/CACNA1) (PubMed:10098851). In GH3 neuroendocrinal cell line, it reversibly inhibits the A-type potassium current but does not block other potassium currents or calcium channels (PubMed:10098851).

Protein Name Kappa-ctenitoxin-Pn1a (Kappa-CNTX-Pn1a) (Neurotoxin Tx3-1) (PNTx3-1) (PhKv)

Venom Taxon Group spider

Protein Family Neurotoxin 02 (plectoxin) family

Protein Existence Evidence at protein level

Keywords Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16278100, ECO:0000305|PubMed:8446961}.

Keywords

Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin

Neurotoxin_Type

Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 02 (plectoxin) family

Protein_Name

Kappa-ctenitoxin-Pn1a (Kappa-CNTX-Pn1a) (Neurotoxin Tx3-1) (PNTx3-1) (PhKv)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16278100, ECO:0000305|PubMed:8446961}.

Venom_Taxon_Group

spider