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Peptide record

TPDB34655

Neurotoxin Toxicity standard
81 amino acids
Basic Information
3D PDB MODEL
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TPDB34655
Neurotoxin Toxicity
Toxicity and safety peptides
Peptipedia
standard
No
A 81-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.
Sequence
MKVAIVFLSLLVLAFASESIEENREEFPVEESARCADINGACKSDCDCCGDSVTCDCYWSDSCKCRESNFKIGMAIRKKFC
Physicochemical Analysis
C383H605N103O124S12
QH
C
9021.35
4.50
10
14
30
-4
19.84
0.004
69.88
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
7615
84.41
24
Residue Composition
number
7
A
4
R
3
N
6
D
10
C
8
E
0
Q
3
G
0
H
5
I
4
L
6
K
2
M
5
F
1
P
9
S
1
T
1
W
1
Y
5
V
Amino Acid Distribution
A: 7 R: 4 N: 3 D: 6 C: 10 E: 8 Q: 0 G: 3 H: 0 I: 5 L: 4 K: 6 M: 2 F: 5 P: 1 S: 9 T: 1 W: 1 Y: 1 V: 5
Chemical Descriptors
81
C383H605N103O124S12
9021.35
4.50
-4
19.84
0.004
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: This insecticidal neurotoxin targets two types of channels/receptors. It reversibly inhibits the N-methyl-D-aspartate (NMDA)-subtype of ionotropic glutamate receptor (GRIN) (PubMed:10978749). It inhibits glutamate uptake from rat brain synaptosomes, and blocks GRIN in hippocampal slices (PubMed:10978749, PubMed:26802625). It also acts on sodium channels of both insects and mammals (PubMed:26747232). On sodium channel insects, it strongly slows down channel inactivation (EC(50)=212.5 nM) and causes an increase (105%) in peak amplitude (at 1 uM) of B.germanica sodium channel (Nav), whereas it inhibits all mammalien sodium channels tested with the following order of potency: Nav1.3/SCN3A (IC(50)=1.5 uM) > Nav1.6/SCN8A > Nav1.5/SCN5A > Nav1.4/SCN4A >= Nav1.2/SCN2A (PubMed:26747232). In vivo, it is highly toxic to house fly (Musca domestica), cockroach (Periplaneta americana), and cricket (Acheta domesticus) (PubMed:10978749). In different rat pain models (induced by PGE2, carrageenan or glutamate), it shows antinociceptive effect that may be related to an inhibitory activity on the glutamatergic system (PubMed:31467512). {ECO:0000269|PubMed:10978749, ECO:0000269|PubMed:26747232, ECO:0000269|PubMed:26802625, ECO:0000269|PubMed:31467512}.

Target

voltage-gated sodium channel (Nav) glutamate receptor

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer)
Neurotoxin Postsynaptic neurotoxin Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Voltage-gated sodium channel impairing toxin
This insecticidal neurotoxin targets two types of channels/receptors. It reversibly inhibits the N-methyl-D-aspartate (NMDA)-subtype of ionotropic glutamate receptor (GRIN) (PubMed:10978749).
GAMMA-ctenitoxin-Pn1a (GAMMA-CNTX-Pn1a) (Insecticidal neurotoxin Tx4(5-5)) (PnTx4(5-5)) (PnTx4-5-5) (Toxin Pn4A)
spider
Neurotoxin 03 (Tx2) family, 05 subfamily
Evidence at protein level
Direct protein sequencing Disulfide bond Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Knottin Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10978749}.
Additional Detail Fields 7 fields
Direct protein sequencing Disulfide bond Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Knottin Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Postsynaptic neurotoxin Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 03 (Tx2) family, 05 subfamily
GAMMA-ctenitoxin-Pn1a (GAMMA-CNTX-Pn1a) (Insecticidal neurotoxin Tx4(5-5)) (PnTx4(5-5)) (PnTx4-5-5) (Toxin Pn4A)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10978749}.
spider