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Peptide record

TPDB34651

Neurotoxin Toxicity standard
87 amino acids
Basic Information
3D PDB MODEL
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TPDB34651
Neurotoxin Toxicity
Toxicity and safety peptides
Peptipedia
standard
No
A 87-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.
Sequence
MKVLVLITLAVLGAMFVWTSAAELEERGSDQRDSPAWVKSMERIFQSEERACREWLGGCSKDADCCAHLECRKKWPYHCVWDWTVRK
Physicochemical Analysis
C447H692N126O126S9
N
AE
10135.72
6.93
15
13
36
-0
17.30
-0.305
72.87
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
34865
343.98
18
Residue Composition
number
8
A
7
R
0
N
5
D
6
C
8
E
2
Q
4
G
2
H
2
I
7
L
6
K
3
M
2
F
2
P
6
S
3
T
6
W
1
Y
7
V
Amino Acid Distribution
A: 8 R: 7 N: 0 D: 5 C: 6 E: 8 Q: 2 G: 4 H: 2 I: 2 L: 7 K: 6 M: 3 F: 2 P: 2 S: 6 T: 3 W: 6 Y: 1 V: 7
Chemical Descriptors
87
C447H692N126O126S9
10135.72
6.93
-0
17.30
-0.305
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Potent and selective inhibitor of hNav1.7/SCN9A (IC(50)=610 nM) (PubMed:29393892). Also shows a weak activity towards Nav1.3/SCN3A (IC(50)=7950 nM) (PubMed:29393892). In addition, inhibits voltage-gated potassium channels (Kv) in rat DRG neurons (PubMed:18581053). It does not alter the voltage dependence of activation, but it causes a small hyperpolarizing shift in the steady-state inactivations of Nav1.7/SNC9A (PubMed:29393892). Chimera experiments show that the toxin binds to the DIIS3-S4 linker (site 4) of Nav1.7/SCN9A, whereas Nav1.7/SCN9A Asp-827 residue is shown by substitution experiments to be critical for its sensitivity (PubMed:19463735, PubMed:29393892). The toxin traps the domain II voltage sensor in the closed configuration, and not in an outward position (PubMed:29393892). In vivo, shows analgesic activity in three rodent pain models (formalin-induced, acid-induced, and thermal) (PubMed:29393892). {ECO:0000269|PubMed:18581053, ECO:0000269|PubMed:29393892, ECO:0000305|PubMed:19463735}.

Target

voltage-gated sodium channel (Nav) voltage-gated potassium channel (Kv)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys jingzhao)
Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Potent and selective inhibitor of hNav1.7/SCN9A (IC(50)=610 nM) (PubMed:29393892). Also shows a weak activity towards Nav1.3/SCN3A (IC(50)=7950 nM) (PubMed:29393892).
Mu-theraphotoxin-Cg1a (Mu-TRTX-Cg1a) (Jingzhaotoxin-34) (JZTX-34) (Peptide F6-25.51)
spider
Neurotoxin 10 (Hwtx-1) family, 39 (Jztx-34) subfamily
Evidence at protein level
3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Potassium channel impairing toxin Secreted Signal Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:17476710}.
Additional Detail Fields 7 fields
3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Potassium channel impairing toxin Secreted Signal Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 10 (Hwtx-1) family, 39 (Jztx-34) subfamily
Mu-theraphotoxin-Cg1a (Mu-TRTX-Cg1a) (Jingzhaotoxin-34) (JZTX-34) (Peptide F6-25.51)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:17476710}.
spider