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Peptide record

TPDB34574

Neurotoxin Toxicity standard
116 amino acids
Basic Information
3D PDB MODEL
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TPDB34574
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 Peptipedia
standard
No
A 116-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.
Sequence
MKMKLLGIILLVSFPFVLGFAGIPIEEGENSVEVGEVERSCINVGDFCDGKKDDCQCCRDNAFCSCSVIFGYKTNCRCEVGTTATSYGICMAKHKCGRQTTCTKPCLSKRCKKNHG
Physicochemical Analysis
C540H873N153O164S17
W
C
12677.82
7.93
18
12
36
+4
25.41
-0.072
67.16
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
3855
30.41
37
Residue Composition
number
4
A
5
R
5
N
5
D
14
C
7
E
2
Q
12
G
2
H
7
I
6
L
11
K
3
M
6
F
3
P
7
S
7
T
0
W
2
Y
8
V
Amino Acid Distribution
A: 4 R: 5 N: 5 D: 5 C: 14 E: 7 Q: 2 G: 12 H: 2 I: 7 L: 6 K: 11 M: 3 F: 6 P: 3 S: 7 T: 7 W: 0 Y: 2 V: 8
Chemical Descriptors
116
C540H873N153O164S17
12677.82
7.93
+4
25.41
-0.072
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: This toxin is a potent and practically irreversible antagonist of both Cav2.1/CACNA1A and Cav2.2/CACNA1B calcium channels, while it displays a partial and rapidly reversible block of Cav2.3/CACNA1E calcium channels and no effect on Cav3/CACNA1 calcium channels. Inhibits glutamate uptake from rat brain synaptosomes by an interaction between cysteines from both glutamate transporter and toxin (PubMed:10923668). Blocks potassium-induced exocytosis of synaptic vesicles in brain cortical synaptosomes (IC(50)=1.1 nM) (PubMed:18524484). In rat brain, inhibits glutamate release, neuronal death and loss of neurotransmission in the hippocampus resulting from ischemia (PubMed:19370546). In vivo, induces rapid general flaccid paralysis followed by death in 10-30 minutes at dose levels of 5 ug per mouse. {ECO:0000269|PubMed:10510308, ECO:0000269|PubMed:10923668, ECO:0000269|PubMed:11397544, ECO:0000269|PubMed:11827974, ECO:0000269|PubMed:18524484, ECO:0000269|PubMed:19370546, ECO:0000269|PubMed:8446961, ECO:0000269|PubMed:9631431, ECO:0000269|PubMed:9683727}.

Target

voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin
This toxin is a potent and practically irreversible antagonist of both Cav2.1/CACNA1A and Cav2.2/CACNA1B calcium channels, while it displays a partial and rapidly reversible block of Cav2.3/CACNA1E calcium channels and no effect on Cav3/CACNA1 calcium channels. Inhibits glutamate uptake from rat brain synaptosomes by an interaction between cysteines from both glutamate transporter and toxin (PubMed:10923668).
Omega-ctenitoxin-Pn3a (Omega-CNTX-Pn3a) (Neurotoxin Tx3-4) (Omega-phonetoxin-2A) (Omega-phonetoxin-IIA) (Omega-Ptx-IIA) (PF3) (Phoneutriatoxin 3-4) (Pn3-4A)
spider
Neurotoxin 04 (omega-agtx) family, 03 (type II/III omega-agtx) subfamily
Evidence at protein level
Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:16278100}.
Additional Detail Fields 7 fields
Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin
Evidence at protein level
Neurotoxin 04 (omega-agtx) family, 03 (type II/III omega-agtx) subfamily
Omega-ctenitoxin-Pn3a (Omega-CNTX-Pn3a) (Neurotoxin Tx3-4) (Omega-phonetoxin-2A) (Omega-phonetoxin-IIA) (Omega-Ptx-IIA) (PF3) (Phoneutriatoxin 3-4) (Pn3-4A)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:16278100}.
spider