TPDB

3D PDB MODEL

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TPDB ID TPDB34246

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 90-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

MKCAVLFLSVIALVHIFVVEAEEEPDSDALVPQERACIPRGEICTDDCECCGCDNQCYCPPGSSLGIFKCSCAHANKYFCNRKKEKCKKA

FASTA

>TPDB34246|Neurotoxin;Toxicity|length=90
MKCAVLFLSVIALVHIFVVEAEEEPDSDALVPQERACIPRGEICTDDCECCGCDNQCYCPPGSSLGIFKCSCAHANKYFCNRKKEKCKKA

Formula C424H674N116O130S14

Absent Amino Acids W

Common Amino Acids C

Mass 9925.56

PI 5.52

Basic Residues 13

Acidic Residues 13

Hydrophobic Residues 31

Net Charge -2

Boman Index 25.04

Hydrophobicity -0.048

Aliphatic Index 71.56

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 3730

Absorbance 280nm 37.58

Polar Residues 26

number

8

A

3

R

3

N

5

D

13

C

8

E

2

Q

4

G

2

H

5

I

5

L

8

K

1

M

4

F

5

P

5

S

1

T

0

W

2

Y

6

V

Amino Acid Distribution

A: 8 R: 3 N: 3 D: 5 C: 13 E: 8 Q: 2 G: 4 H: 2 I: 5 L: 5 K: 8 M: 1 F: 4 P: 5 S: 5 T: 1 W: 0 Y: 2 V: 6

Length

90

Formula

C424H674N116O130S14

Mass

9925.56

PI

5.52

Net Charge

-2

Boman Index

25.04

Hydrophobicity

-0.048

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Potent blocker of nociceptor cation channels TRPA1 and high voltage-activated calcium channels (PubMed:12470700, PubMed:15933156, PubMed:27759880). It acts mainly on P/Q-type (Cav2.1/CACNA1A) calcium channels and has a minor effect on L- (Cav1/CACNA1) and N-type (Cav2.2/CACNA1B) calcium channels (PubMed:12470700, PubMed:15933156). Blocks glutamate release in synaptic transmission mediated by calcium channels (PubMed:12470700). The toxin also inhibits the KCl-induced increase of intrasynaptosomal free calcium (PubMed:12470700). It also shows antiproliferative effects on different glioma cell lines (M059J, U-138MG and U-251MG) (PubMed:28202361). In vivo, it shows analgesic effects in rodent models of pain (PubMed:18774645). In addition, it selectively blocks nocifensive responses evoked by reactive TRPA1 channel agonist (PubMed:27759880). Furthermore, it also reduces the TRPA1 channel-dependent hyperalgesia in a model of neuropathic pain induced by the chemotherapeutic agent BTZ (PubMed:27759880). In addition, it causes reduction of tumor area in the mouse glioma model, that is induced by the orthotopic injection of GL261 cells into the brain (PubMed:28202361). {ECO:0000269|PubMed:12470700, ECO:0000269|PubMed:15933156, ECO:0000269|PubMed:18774645, ECO:0000269|PubMed:28202361}.

Target

Target or Channel voltage-gated calcium channel (Cav) TRP channel

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer)

Neurotoxin Type Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Mechanism

Potent blocker of nociceptor cation channels TRPA1 and high voltage-activated calcium channels (PubMed:12470700, PubMed:15933156, PubMed:27759880). It acts mainly on P/Q-type (Cav2.1/CACNA1A) calcium channels and has a minor effect on L- (Cav1/CACNA1) and N-type (Cav2.2/CACNA1B) calcium channels (PubMed:12470700, PubMed:15933156).

Protein Name Omega-ctenitoxin-Pn4a (Omega-CNTX-Pn4a) (CTK 01512-2) (Neurotoxin Tx3-6) (PnTx3-6) (Ph-alpha-1-beta toxin)

Venom Taxon Group spider

Protein Family Neurotoxin 09 (Tx3-6) family

Protein Existence Evidence at protein level

Keywords 3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16278100, ECO:0000305|PubMed:8446961}.

Keywords

3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin

Neurotoxin_Type

Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 09 (Tx3-6) family

Protein_Name

Omega-ctenitoxin-Pn4a (Omega-CNTX-Pn4a) (CTK 01512-2) (Neurotoxin Tx3-6) (PnTx3-6) (Ph-alpha-1-beta toxin)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16278100, ECO:0000305|PubMed:8446961}.

Venom_Taxon_Group

spider