TPDB

3D PDB MODEL

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TPDB ID TPDB33518

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 66-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

LTCVTSKSIFGITTENCPDGQNLCFKKWYYLNHRYSDITWGCAATCPKPTNVRETIHCCETDKCNE

FASTA

>TPDB33518|Neurotoxin;Toxicity|length=66
LTCVTSKSIFGITTENCPDGQNLCFKKWYYLNHRYSDITWGCAATCPKPTNVRETIHCCETDKCNE

Formula C326H499N89O102S8

Absent Amino Acids M

Common Amino Acids T

Mass 7553.56

PI 6.85

Basic Residues 9

Acidic Residues 7

Hydrophobic Residues 18

Net Charge -0

Boman Index 12.15

Hydrophobicity -0.512

Aliphatic Index 53.18

Half Life

Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 15970

Absorbance 280nm 211.42

Polar Residues 29

number

2

A

2

R

5

N

3

D

8

C

4

E

1

Q

3

G

2

H

4

I

3

L

5

K

0

M

2

F

3

P

3

S

9

T

2

W

3

Y

2

V

Amino Acid Distribution

A: 2 R: 2 N: 5 D: 3 C: 8 E: 4 Q: 1 G: 3 H: 2 I: 4 L: 3 K: 5 M: 0 F: 2 P: 3 S: 3 T: 9 W: 2 Y: 3 V: 2

Length

66

Formula

C326H499N89O102S8

Mass

7553.56

PI

6.85

Net Charge

-0

Boman Index

12.15

Hydrophobicity

-0.512

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Selectively binds with high-affinity to the a2B-adrenoceptor subtype (ADRA2B) (PubMed:20466015). The toxin reversibly binds to ADRA2B, and its mode of inhibition is non-competitive (PubMed:20466015). The toxin has also been described to bind with high affinity to all muscarinic receptor subtypes (Ki=23 nM (on CHRM1), Ki=44 nM (on CHRM2), Ki=3 nM (on CHRM3), Ki=5 nM (on CHRM4), and Ki=8 nM (on CHRM5)) but no other data support these affinity values (PubMed:8536706). {ECO:0000269|PubMed:10978757, ECO:0000269|PubMed:20466015, ECO:0000269|PubMed:21557730, ECO:0000269|PubMed:8536706}.

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Dendroaspis polylepis polylepis (Black mamba)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin G-protein coupled receptor impairing toxin

Mechanism

Selectively binds with high-affinity to the a2B-adrenoceptor subtype (ADRA2B) (PubMed:20466015). The toxin reversibly binds to ADRA2B, and its mode of inhibition is non-competitive (PubMed:20466015).

Protein Name Muscarinic toxin alpha (MT-alpha)

Venom Taxon Group snake

Protein Family Three-finger toxin family, Short-chain subfamily, Aminergic toxin sub-subfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Direct protein sequencing Disulfide bond G-protein coupled receptor impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.

Keywords

3D-structure Direct protein sequencing Disulfide bond G-protein coupled receptor impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin G-protein coupled receptor impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Three-finger toxin family, Short-chain subfamily, Aminergic toxin sub-subfamily

Protein_Name

Muscarinic toxin alpha (MT-alpha)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.

Venom_Taxon_Group

snake