TPDB

3D PDB MODEL

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TPDB ID TPDB33217

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 57-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

LICHRVHGLQTCEPDQKFCFRKTTMFFPNHPVLLMGCTSSCPTEKYSVCCSTDKCNK

FASTA

>TPDB33217|Neurotoxin;Toxicity|length=57
LICHRVHGLQTCEPDQKFCFRKTTMFFPNHPVLLMGCTSSCPTEKYSVCCSTDKCNK

Formula C280H440N78O81S10

Absent Amino Acids AW

Common Amino Acids C

Mass 6515.65

PI 8.10

Basic Residues 10

Acidic Residues 4

Hydrophobic Residues 15

Net Charge +3

Boman Index 10.95

Hydrophobicity -0.258

Aliphatic Index 49.47

Half Life

Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1990

Absorbance 280nm 30.54

Polar Residues 23

number

0

A

2

R

2

N

2

D

8

C

2

E

2

Q

2

G

3

H

1

I

4

L

5

K

2

M

4

F

4

P

4

S

6

T

0

W

1

Y

3

V

Amino Acid Distribution

A: 0 R: 2 N: 2 D: 2 C: 8 E: 2 Q: 2 G: 2 H: 3 I: 1 L: 4 K: 5 M: 2 F: 4 P: 4 S: 4 T: 6 W: 0 Y: 1 V: 3

Length

57

Formula

C280H440N78O81S10

Mass

6515.65

PI

8.10

Net Charge

+3

Boman Index

10.95

Hydrophobicity

-0.258

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: This toxin binds and inhibits rat muscle adult alpha-1-beta-1-delta-epsilon/CHRNA1-CHRNB1-CHRND-CHRNE (IC(50)=3.1 uM) and fetal alpha-1-beta-1-gamma-delta/CHRNA1-CHRNB1-CHRNG-CHRND (IC(50)=5.6 uM) nicotinic acetylcholine receptors (nAChR) (PubMed:26448325). Shows a very low inhibition on rat neuronal alpha-3-beta-2/CHRNA3-CHRNB2 nAChR (IC(50)=50.2 uM) nAChR (PubMed:26448325). Binds to the acetylcholine-binding pocket and acts as a competitive antagonist (PubMed:26448325). Does not inhibit human glycine receptor (homopentamer composed of alpha-1 subunits, GLRA1), but seems to potentiate it (about 2-fold increased activity) (PubMed:26448325). {ECO:0000269|PubMed:12056805, ECO:0000269|PubMed:26448325}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Ophiophagus hannah (King cobra) (Naja hannah)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Mechanism

This toxin binds and inhibits rat muscle adult alpha-1-beta-1-delta-epsilon/CHRNA1-CHRNB1-CHRND-CHRNE (IC(50)=3.1 uM) and fetal alpha-1-beta-1-gamma-delta/CHRNA1-CHRNB1-CHRNG-CHRND (IC(50)=5.6 uM) nicotinic acetylcholine receptors (nAChR) (PubMed:26448325). Shows a very low inhibition on rat neuronal alpha-3-beta-2/CHRNA3-CHRNB2 nAChR (IC(50)=50.2 uM) nAChR (PubMed:26448325).

Protein Name Neurotoxin Oh9-1 (OMEGA-neurotoxin Oh9-1) (Three-finger toxin) (3FTx)

Venom Taxon Group snake

Protein Family Three-finger toxin family, Short-chain subfamily

Protein Existence Evidence at protein level

Keywords Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:12056805}.

Keywords

Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Three-finger toxin family, Short-chain subfamily

Protein_Name

Neurotoxin Oh9-1 (OMEGA-neurotoxin Oh9-1) (Three-finger toxin) (3FTx)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:12056805}.

Venom_Taxon_Group

snake