TPDB

3D PDB MODEL

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TPDB ID TPDB32747

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 46-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

KECMTDGTVCYIHNHNDCCGSCLCSNGPIARPWEMMVGNCMCGPKA

FASTA

>TPDB32747|Neurotoxin;Toxicity|length=46
KECMTDGTVCYIHNHNDCCGSCLCSNGPIARPWEMMVGNCMCGPKA

Formula C201H316N60O64S12

Absent Amino Acids QF

Common Amino Acids C

Mass 4981.80

PI 6.01

Basic Residues 5

Acidic Residues 4

Hydrophobic Residues 13

Net Charge -1

Boman Index 7.82

Hydrophobicity -0.137

Aliphatic Index 42.39

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 7490

Absorbance 280nm 150.35

Polar Residues 17

number

2

A

1

R

4

N

2

D

8

C

2

E

0

Q

5

G

2

H

2

I

1

L

2

K

4

M

0

F

3

P

2

S

2

T

1

W

1

Y

2

V

Amino Acid Distribution

A: 2 R: 1 N: 4 D: 2 C: 8 E: 2 Q: 0 G: 5 H: 2 I: 2 L: 1 K: 2 M: 4 F: 0 P: 3 S: 2 T: 2 W: 1 Y: 1 V: 2

Length

46

Formula

C201H316N60O64S12

Mass

4981.80

PI

6.01

Net Charge

-1

Boman Index

7.82

Hydrophobicity

-0.137

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Insecticidal toxin (PubMed:11689233). It inhibits insect voltage-gated sodium channels (Nav) by partially blocking the channel pore (1 uM inhibits 55.7% sodium current in DUM neurons from the American cockroach), not by acting as a gating modifier (PubMed:25559770). The inhibition is only partially reversible (about 25% recovery) after prolonged washout (PubMed:25559770). In vivo, the toxin causes flaccid paralysis followed by death when injected into Heliothis virescens larvae (PubMed:11689233). It also causes uncoordinated movements followed by full paralysis to sheep blowflies (Lucilia cuprina) (PubMed:25559770). {ECO:0000269|PubMed:11689233, ECO:0000269|PubMed:25559770}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Segestria florentina (Tube-web spider) (Segestria gracilis)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Insecticidal toxin (PubMed:11689233). It inhibits insect voltage-gated sodium channels (Nav) by partially blocking the channel pore (1 uM inhibits 55.7% sodium current in DUM neurons from the American cockroach), not by acting as a gating modifier (PubMed:25559770).

Protein Name Mu-segestritoxin-Sf1a (Mu-SGTX-Sf1a) (F5.6) (Toxin SFI1)

Venom Taxon Group spider

Protein Family Neurotoxin 16 (SFI) family

Protein Existence Evidence at protein level

Keywords 3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:11689233}.

Keywords

3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 16 (SFI) family

Protein_Name

Mu-segestritoxin-Sf1a (Mu-SGTX-Sf1a) (F5.6) (Toxin SFI1)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:11689233}.

Venom_Taxon_Group

spider