TPDB

3D PDB MODEL

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TPDB ID TPDB32495

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 17-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, and ToxinPred 3.0, with an available 3D structural model.

Sequence

IVRRACCSDRRCRWRCG

FASTA

>TPDB32495|Neurotoxin;Toxicity|length=17
IVRRACCSDRRCRWRCG

Formula C82H142N36O21S4

Absent Amino Acids NEQHLKMFPTY

Common Amino Acids R

Mass 2096.50

PI 10.68

Basic Residues 6

Acidic Residues 1

Hydrophobic Residues 4

Net Charge +5

Boman Index 3.01

Hydrophobicity -0.712

Aliphatic Index 45.88

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 5750

Absorbance 280nm 274.27

Polar Residues 5

number

1

A

6

R

0

N

1

D

4

C

0

E

0

Q

1

G

0

H

1

I

0

L

0

K

0

M

0

F

0

P

1

S

0

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 1 R: 6 N: 0 D: 1 C: 4 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 0 K: 0 M: 0 F: 0 P: 0 S: 1 T: 0 W: 1 Y: 0 V: 1

Length

17

Formula

C82H142N36O21S4

Mass

2096.50

PI

10.68

Net Charge

+5

Boman Index

3.01

Hydrophobicity

-0.712

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks neuronal mammalian alpha-7 (human and rat) and alpha-3/beta-2 (human) and muscle alpha-1-beta-1-delta-epsilon (human) nAChRs (PubMed:12384509, PubMed:15609996). Acts voltage-independently (PubMed:12384509). Does not compete with alpha-bungarotoxin for binding to the receptor (PubMed:12384509, PubMed:15609996). Binds to a different site than alpha-conotoxin ImI (PubMed:15609996). {ECO:0000269|PubMed:12384509, ECO:0000269|PubMed:15609996}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor proton-gated sodium channel / ASIC

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus imperialis (Imperial cone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Mechanism

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks neuronal mammalian alpha-7 (human and rat) and alpha-3/beta-2 (human) and muscle alpha-1-beta-1-delta-epsilon (human) nAChRs (PubMed:12384509, PubMed:15609996).

Protein Name Alpha-conotoxin ImII (Alpha-CTx ImII)

Venom Taxon Group cone snail

Protein Family Conotoxin A superfamily

Protein Existence Evidence at protein level

Keywords Acetylcholine receptor inhibiting toxin Amidation Cleavage on pair of basic residues Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305}.

Keywords

Acetylcholine receptor inhibiting toxin Amidation Cleavage on pair of basic residues Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin A superfamily

Protein_Name

Alpha-conotoxin ImII (Alpha-CTx ImII)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305}.

Venom_Taxon_Group

cone snail