TPDB

3D PDB MODEL

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TPDB ID TPDB32328

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 38-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

ILSLSLLDRSTRCKGTGKSCSRIAYNCCTGSCRSGKCG

FASTA

>TPDB32328|Neurotoxin;Toxicity|length=38
ILSLSLLDRSTRCKGTGKSCSRIAYNCCTGSCRSGKCG

Formula C159H278N54O53S6

Absent Amino Acids EQHMFPWV

Common Amino Acids S

Mass 3986.65

PI 9.29

Basic Residues 7

Acidic Residues 1

Hydrophobic Residues 8

Net Charge +6

Boman Index 3.83

Hydrophobicity -0.176

Aliphatic Index 64.21

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 1865

Absorbance 280nm 46.78

Polar Residues 18

number

1

A

4

R

1

N

1

D

6

C

0

E

0

Q

5

G

0

H

2

I

4

L

3

K

0

M

0

F

0

P

7

S

3

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 1 R: 4 N: 1 D: 1 C: 6 E: 0 Q: 0 G: 5 H: 0 I: 2 L: 4 K: 3 M: 0 F: 0 P: 0 S: 7 T: 3 W: 0 Y: 1 V: 0

Length

38

Formula

C159H278N54O53S6

Mass

3986.65

PI

9.29

Net Charge

+6

Boman Index

3.83

Hydrophobicity

-0.176

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This peptide reversibly and selectively inhibits Cav2.2/CACNA1B (IC(50)=11.5 nM) voltage-gated calcium channels. Channel time recovery after toxin exposure is short (about 50 seconds). In vivo, it effectively and dose-dependently reduces nociceptive behavior in the formalin test and in neuropathic pain models, and reduces mechanical and thermal allodynia in the tail nerve injury rat model. It also shows significant analgesic effects on writhing in mouse neurotransmitter- and cytokine-induced pain models, though it has no effect on acute thermal pain and interferon-gamma-induced pain. It also depresses blood pressure immediately after administration, but pressure recovers relatively quickly and completely. {ECO:0000269|PubMed:21172037}.

Target

Target or Channel voltage-gated calcium channel (Cav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus fulmen (Thunderbolt cone) (Chelyconus fulmen)

Neurotoxin Type Neurotoxin Presynaptic neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Mechanism

Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This peptide reversibly and selectively inhibits Cav2.2/CACNA1B (IC(50)=11.5 nM) voltage-gated calcium channels.

Protein Name Conotoxin FVIA

Venom Taxon Group cone snail

Protein Family Conotoxin O1 superfamily

Protein Existence Evidence at protein level

Keywords Amidation Calcium channel impairing toxin Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Presynaptic neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:21172037}.

Keywords

Amidation Calcium channel impairing toxin Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Presynaptic neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin

Neurotoxin_Type

Neurotoxin Presynaptic neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin O1 superfamily

Protein_Name

Conotoxin FVIA

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:21172037}.

Venom_Taxon_Group

cone snail