TPDB

3D PDB MODEL

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TPDB ID TPDB32046

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 47-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

GVPCLCDSDGPSVRGNTLSGIIWLAGCPSGWHNCKKHGPTIGWCCKQ

FASTA

>TPDB32046|Neurotoxin;Toxicity|length=47
GVPCLCDSDGPSVRGNTLSGIIWLAGCPSGWHNCKKHGPTIGWCCKQ

Formula C213H329N63O61S6

Absent Amino Acids EMFY

Common Amino Acids G

Mass 4940.70

PI 8.02

Basic Residues 6

Acidic Residues 2

Hydrophobic Residues 12

Net Charge +2

Boman Index 2.63

Hydrophobicity -0.147

Aliphatic Index 64.26

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 16875

Absorbance 280nm 341.55

Polar Residues 15

number

1

A

1

R

2

N

2

D

6

C

0

E

1

Q

8

G

2

H

3

I

3

L

3

K

0

M

0

F

4

P

4

S

2

T

3

W

0

Y

2

V

Amino Acid Distribution

A: 1 R: 1 N: 2 D: 2 C: 6 E: 0 Q: 1 G: 8 H: 2 I: 3 L: 3 K: 3 M: 0 F: 0 P: 4 S: 4 T: 2 W: 3 Y: 0 V: 2

Length

47

Formula

C213H329N63O61S6

Mass

4940.70

PI

8.02

Net Charge

+2

Boman Index

2.63

Hydrophobicity

-0.147

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation. Has a strong effect on crustaceans and insects (DmNav1) and a weaker effect on mammals. This toxin is highly potent at mammalian Nav1.1/SCN1A (EC(50)=6.01 nM) and Nav1.2/SCN2A (EC(50)=7.88 nM) (PubMed:15169781). It also has great activity on Nav1.5/SCN5A (EC(50)=49.05 nM), Nav1.4/SCN4A (EC(50)=109.49 nM) and Nav1.6/SCN8A (EC(50)=about 180 nM) and is less potent on Nav1.3/SCN3A (EC(50)=759.22 nM) (when measured as the increase in the slow component) (PubMed:15169781). {ECO:0000269|PubMed:15169781, ECO:0000269|PubMed:8660409, ECO:0000269|PubMed:9236205}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Anemonia sulcata (Mediterranean snakelocks sea anemone)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation. Has a strong effect on crustaceans and insects (DmNav1) and a weaker effect on mammals.

Protein Name Delta-actitoxin-Avd1c (Delta-AITX-Avd1c) (ATX-II) (ATX II) (Anemonia sulcata toxin 2) (As2) (Neurotoxin 2) (Toxin II)

Venom Taxon Group sea anemone

Protein Family Sea anemone sodium channel inhibitory toxin family, Type I subfamily

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Sea anemone sodium channel inhibitory toxin family, Type I subfamily

Protein_Name

Delta-actitoxin-Avd1c (Delta-AITX-Avd1c) (ATX-II) (ATX II) (Anemonia sulcata toxin 2) (As2) (Neurotoxin 2) (Toxin II)

Venom_Taxon_Group

sea anemone