TPDB

3D PDB MODEL

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TPDB ID TPDB32022

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 39-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

GVDKEGCRKLLGGCTIDDDCCPHLGCNKKYWHCGWDGTF

FASTA

>TPDB32022|Neurotoxin;Toxicity|length=39
GVDKEGCRKLLGGCTIDDDCCPHLGCNKKYWHCGWDGTF

Formula C185H277N53O56S6

Absent Amino Acids AQMS

Common Amino Acids G

Mass 4331.92

PI 6.02

Basic Residues 7

Acidic Residues 6

Hydrophobic Residues 9

Net Charge -1

Boman Index 6.95

Hydrophobicity -0.564

Aliphatic Index 47.44

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 12865

Absorbance 280nm 296.98

Polar Residues 10

number

0

A

1

R

1

N

5

D

6

C

1

E

0

Q

7

G

2

H

1

I

3

L

4

K

0

M

1

F

1

P

0

S

2

T

2

W

1

Y

1

V

Amino Acid Distribution

A: 0 R: 1 N: 1 D: 5 C: 6 E: 1 Q: 0 G: 7 H: 2 I: 1 L: 3 K: 4 M: 0 F: 1 P: 1 S: 0 T: 2 W: 2 Y: 1 V: 1

Length

39

Formula

C185H277N53O56S6

Mass

4331.92

PI

6.02

Net Charge

-1

Boman Index

6.95

Hydrophobicity

-0.564

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Inhibits mammalian voltage-gated sodium channel subtypes Nav1.5/SCN5A and Nav1.8/SCN10A by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current. In vivo, this toxin causes erect, elevated tail, initial partial ataxia, followed by recovery over approximately 1 hour after injection and the progressive development of shaking. Although paralysis subsides, the body tremors never cease and persist until the end of the experiment. {ECO:0000269|PubMed:16267209}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Ceratogyrus marshalli (Straighthorned baboon tarantula) (Ceratogyrus cornuatus)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Inhibits mammalian voltage-gated sodium channel subtypes Nav1.5/SCN5A and Nav1.8/SCN10A by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current. In vivo, this toxin causes erect, elevated tail, initial partial ataxia, followed by recovery over approximately 1 hour after injection and the progressive development of shaking.

Protein Name Beta-theraphotoxin-Cm2a (Beta-TRTX-Cm2a) (Ceratotoxin-3) (CcoTx3)

Venom Taxon Group spider

Protein Family Neurotoxin 10 (Hwtx-1) family, 47 subfamily

Protein Existence Evidence at protein level

Keywords Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16267209}.

Keywords

Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 10 (Hwtx-1) family, 47 subfamily

Protein_Name

Beta-theraphotoxin-Cm2a (Beta-TRTX-Cm2a) (Ceratotoxin-3) (CcoTx3)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16267209}.

Venom_Taxon_Group

spider