TPDB

3D PDB MODEL

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TPDB ID TPDB32001

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 48-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

GVACRCDSDGPTVRGDSLSGTLWLTGGCPSGWHNCRGSGPFIGYCCKK

FASTA

>TPDB32001|Neurotoxin;Toxicity|length=48
GVACRCDSDGPTVRGDSLSGTLWLTGGCPSGWHNCRGSGPFIGYCCKK

Formula C209H322N64O65S6

Absent Amino Acids EQM

Common Amino Acids G

Mass 4963.61

PI 8.02

Basic Residues 6

Acidic Residues 3

Hydrophobic Residues 11

Net Charge +2

Boman Index 2.77

Hydrophobicity -0.263

Aliphatic Index 46.67

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 12865

Absorbance 280nm 259.19

Polar Residues 16

number

1

A

3

R

1

N

3

D

6

C

0

E

0

Q

10

G

1

H

1

I

3

L

2

K

0

M

1

F

3

P

5

S

3

T

2

W

1

Y

2

V

Amino Acid Distribution

A: 1 R: 3 N: 1 D: 3 C: 6 E: 0 Q: 0 G: 10 H: 1 I: 1 L: 3 K: 2 M: 0 F: 1 P: 3 S: 5 T: 3 W: 2 Y: 1 V: 2

Length

48

Formula

C209H322N64O65S6

Mass

4963.61

PI

8.02

Net Charge

+2

Boman Index

2.77

Hydrophobicity

-0.263

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Binds to the sodium channels Nav1.1/SCN1A (EC(50)=165 nM), Nav1.5/SCN5A (EC(50)=103 nM) and Nav1.6/SCN8A (EC(50)=133 nM), thereby delaying their inactivation (PubMed:18342901, PubMed:21802465). Also inhibits Nav1.2/SCN2A, Nav1.3/SCN3A, and Nav1.4/SCN4A, but to a lesser extent (PubMed:21802465). Inhibits Nav1.5 differently from isoforms Nav1.1 and Nav1.6. In Nav1.5 the effect consists in a right-shift of inactivation whereas in both Nav1.1 and Nav1.6 the effect consists in an incomplete inactivation (PubMed:21802465). {ECO:0000269|PubMed:18342901}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Bunodosoma cangicum (Sea anemone)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Binds to the sodium channels Nav1.1/SCN1A (EC(50)=165 nM), Nav1.5/SCN5A (EC(50)=103 nM) and Nav1.6/SCN8A (EC(50)=133 nM), thereby delaying their inactivation (PubMed:18342901, PubMed:21802465). Also inhibits Nav1.2/SCN2A, Nav1.3/SCN3A, and Nav1.4/SCN4A, but to a lesser extent (PubMed:21802465).

Protein Name Delta-actitoxin-Bcg1b (Delta-AITX-Bcg1b) (Cangitoxin II) (Cangitoxin-2) (Cangitoxin-II) (CGTX-II)

Venom Taxon Group sea anemone

Protein Family Sea anemone sodium channel inhibitory toxin family, Type I subfamily

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Sea anemone sodium channel inhibitory toxin family, Type I subfamily

Protein_Name

Delta-actitoxin-Bcg1b (Delta-AITX-Bcg1b) (Cangitoxin II) (Cangitoxin-2) (Cangitoxin-II) (CGTX-II)

Venom_Taxon_Group

sea anemone