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Peptide record

TPDB31989

Neurotoxin Toxicity standard
41 amino acids
Basic Information
3D PDB MODEL
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TPDB31989
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 Peptipedia
standard
No
A 41-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.
Sequence
GTPCDCYGYTGVYWFMLSRCPSGYGYNLSCHYFMGICCVKR
Physicochemical Analysis
C210H298N52O56S8
AEQ
CGY
4703.47
8.01
4
1
16
+2
-6.09
0.090
42.68
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
14815
314.98
18
Residue Composition
number
0
A
2
R
1
N
1
D
6
C
0
E
0
Q
6
G
1
H
1
I
2
L
1
K
2
M
2
F
2
P
3
S
2
T
1
W
6
Y
2
V
Amino Acid Distribution
A: 0 R: 2 N: 1 D: 1 C: 6 E: 0 Q: 0 G: 6 H: 1 I: 1 L: 2 K: 1 M: 2 F: 2 P: 2 S: 3 T: 2 W: 1 Y: 6 V: 2
Chemical Descriptors
41
C210H298N52O56S8
4703.47
8.01
+2
-6.09
0.090
Evidence Records 2 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Toxin with different activities on acid-sensing ion channels (ASIC) and nicotinic acetylcholine receptors (By similarity) (PubMed:32326130). Is able to bind T.californica muscle-type nicotinic acetylcholine receptors (nAChR) (alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE)), and human alpha-7/CHRNA7 nicotinic acetylcholine receptors (By similarity). Weakly and reversibly inhibits rat homomeric ASIC1 (isoform ASIC1a) (IC(50)=1.25 uM), while it potentiates rat homomeric ASIC3 (EC(50)=1.53 uM) (By similarity) (PubMed:29684594, PubMed:32326130). Rat ASIC1a current inhibition is not complete, and reaches a maximum of 86% inhibition (PubMed:32326130). On rat ASIC3, does not activate the channel itself, but produces a remarkable potentiation of the transient current resulting from the acidic pulse (PubMed:32326130). At the maximal applied concentration, it elicits responses that are twice as high as those produced by extracellular protons (PubMed:32326130). Surprisingly, shows a different activity on human ASIC3. On the truncated human ASIC3 (ASIC3-D20), the toxin weakly inhibits the channel (By similarity). Molecular modeling interaction with rat ASIC1a suggests that it hinders the collapse of acidic pockets and stabilizes nonconducting channels state (PubMed:32326130). In vivo, causes an anxiolytic effect on mouse behavior (By similarity). Also shows an analgesic activity in an acid-induced muscle pain model, and important anti-inflammatory effect in models of acute local inflammation (By similarity). {ECO:0000250|UniProtKB:P0DRI4, ECO:0000269|PubMed:29684594, ECO:0000269|PubMed:32326130}.

Target

nicotinic acetylcholine receptor (nAChR) acetylcholine receptor voltage-gated sodium channel (Nav) proton-gated sodium channel / ASIC

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Radianthus crispa (Leathery sea anemone) (Heteractis crispa)
Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin Proton-gated sodium channel impairing toxin
Toxin with different activities on acid-sensing ion channels (ASIC) and nicotinic acetylcholine receptors (By similarity) (PubMed:32326130). Is able to bind T.californica muscle-type nicotinic acetylcholine receptors (nAChR) (alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE)), and human alpha-7/CHRNA7 nicotinic acetylcholine receptors (By similarity).
Pi-stichotoxin-Hcr5d (Pi-SHTX-Hcr5d) (APETx-like peptide) (Hcr 1b-4)
sea anemone
Sea anemone type 3 (BDS) potassium channel toxin family
Evidence at protein level
Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Postsynaptic neurotoxin Proton-gated sodium channel impairing toxin Secreted Toxin
Evidence 2 Activity

Activity

Neurotoxin
FUNCTION: Toxin with different activities on acid-sensing ion channels (ASIC) and nicotinic acetylcholine receptors (By similarity) (PubMed:36287966). Is able to bind T.californica muscle-type nicotinic acetylcholine receptors (nAChR) (alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE)), and human alpha-7/CHRNA7 nicotinic acetylcholine receptors (PubMed:36287966). Weakly and reversibly inhibits rat homomeric ASIC1 (isoform ASIC1a) (IC(50)=1.25 uM), while it potentiates rat homomeric ASIC3 (EC(50)=1.53 uM) (By similarity) (PubMed:37235375). Rat ASIC1a current inhibition is not complete, and reaches a maximum of 86% inhibition (By similarity). On rat ASIC3, does not activate the channel itself, but produces a remarkable potentiation of the transient current resulting from the acidic pulse (By similarity). At the maximal applied concentration, elicits responses that are twice as high as those produced by extracellular protons (By similarity). Surprisingly, shows a different activity on human ASIC3. On the truncated human ASIC3 (ASIC3-D20), the toxin weakly inhibits the channel (PubMed:37235375). Molecular modeling interaction with rat ASIC1a suggests that it hinders the collapse of acidic pockets and stabilizes nonconducting channels state (By similarity). In vivo, causes an anxiolytic effect on mouse behavior (PubMed:37235375). Also shows an analgesic activity in an acid-induced muscle pain model, and important anti-inflammatory effect in models of acute local inflammation (PubMed:37235375). {ECO:0000250|UniProtKB:C0HL54, ECO:0000269|PubMed:36287966, ECO:0000269|PubMed:37235375}.

Target

nicotinic acetylcholine receptor (nAChR) acetylcholine receptor voltage-gated sodium channel (Nav) proton-gated sodium channel / ASIC

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Heteractis magnifica (Magnificent sea anemone) (Radianthus magnifica)
Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin Proton-gated sodium channel impairing toxin
Toxin with different activities on acid-sensing ion channels (ASIC) and nicotinic acetylcholine receptors (By similarity) (PubMed:36287966). Is able to bind T.californica muscle-type nicotinic acetylcholine receptors (nAChR) (alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE)), and human alpha-7/CHRNA7 nicotinic acetylcholine receptors (PubMed:36287966).
Pi-stichotoxin-Hmg5c (Pi-SHTX-Hmg5c) (APETx-like peptide) (Hmg 1b-4)
sea anemone
Sea anemone type 3 (BDS) potassium channel toxin family
Evidence at protein level
Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Postsynaptic neurotoxin Proton-gated sodium channel impairing toxin Secreted Toxin
Additional Detail Fields 6 fields
Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Postsynaptic neurotoxin Proton-gated sodium channel impairing toxin Secreted Toxin
Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin Proton-gated sodium channel impairing toxin
Evidence at protein level
Sea anemone type 3 (BDS) potassium channel toxin family
Pi-stichotoxin-Hcr5d (Pi-SHTX-Hcr5d) (APETx-like peptide) (Hcr 1b-4) Pi-stichotoxin-Hmg5c (Pi-SHTX-Hmg5c) (APETx-like peptide) (Hmg 1b-4)
sea anemone