TPDB

3D PDB MODEL

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TPDB ID TPDB30951

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

modified_plain

Has_Modification Yes

Description A 34-aa modified multi-activity (Neurotoxin and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Modification

Modified, details unspecified

Sequence

GCANAYKSCNGPHTCCWGYNGYKKACICSGXNWK

FASTA

>TPDB30951|Neurotoxin;Toxicity|length=34
GCANAYKSCNGPHTCCWGYNGYKKACICSGXNWK

Formula C153H223N45O44S6

Absent Amino Acids RDEQLMFV

Common Amino Acids C

Mass 3589.09

PI 8.57

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 9

Net Charge +4

Boman Index -1.28

Hydrophobicity -0.574

Aliphatic Index 20.29

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 15845

Absorbance 280nm 441.48

Polar Residues 16

number

3

A

0

R

4

N

0

D

6

C

0

E

0

Q

5

G

1

H

1

I

0

L

4

K

0

M

0

F

1

P

2

S

1

T

2

W

3

Y

0

V

Amino Acid Distribution

A: 3 R: 0 N: 4 D: 0 C: 6 E: 0 Q: 0 G: 5 H: 1 I: 1 L: 0 K: 4 M: 0 F: 0 P: 1 S: 2 T: 1 W: 2 Y: 3 V: 0

Length

34

Formula

C153H223N45O44S6

Mass

3589.09

PI

8.57

Net Charge

+4

Boman Index

-1.28

Hydrophobicity

-0.574

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Inhibits all known high-voltage activated calcium channels (L-, P/Q- and R-type currents) (Cav), and most effectively the P/Q- (Cav2.1/CACNA1A) and R-type (Cav2.3/CACNA1E) currents (PubMed:10884557). In rat brain, inhibits glutamate release, neuronal death and loss of neurotransmission in the hippocampus resulting from ischemia. In vivo, induces rapid general flaccid paralysis followed by death in 10-30 minutes at dose levels of 5 ug per mouse. {ECO:0000269|PubMed:10884557, ECO:0000269|PubMed:11397544, ECO:0000269|PubMed:19370546, ECO:0000269|PubMed:8660275, ECO:0000269|PubMed:9351520, ECO:0000269|PubMed:9631431}.

Target

Target or Channel voltage-gated calcium channel (Cav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer)

Neurotoxin Type Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Mechanism

Inhibits all known high-voltage activated calcium channels (L-, P/Q- and R-type currents) (Cav), and most effectively the P/Q- (Cav2.1/CACNA1A) and R-type (Cav2.3/CACNA1E) currents (PubMed:10884557). In rat brain, inhibits glutamate release, neuronal death and loss of neurotransmission in the hippocampus resulting from ischemia.

Protein Name Omega-ctenitoxin-Pn2a (Omega-CNTX-Pn2a) (Neurotoxin Tx3-3) (Omega-PnTx3-3) (PnTx3-3)

Venom Taxon Group spider

Protein Family Neurotoxin 02 (plectoxin) family, 01 (Tx3) subfamily

Protein Existence Evidence at protein level

Keywords Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16278100}.

Keywords

Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin

Neurotoxin_Type

Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 02 (plectoxin) family, 01 (Tx3) subfamily

Protein_Name

Omega-ctenitoxin-Pn2a (Omega-CNTX-Pn2a) (Neurotoxin Tx3-3) (Omega-PnTx3-3) (PnTx3-3)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16278100}.

Venom_Taxon_Group

spider