TPDB

3D PDB MODEL

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TPDB ID TPDB30900

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 48-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

GASCRCDSDGPTSRGNTLTGTLWLIGRCPSGWHNCRGSGPFIGYCCKQ

FASTA

>TPDB30900|Neurotoxin;Toxicity|length=48
GASCRCDSDGPTSRGNTLTGTLWLIGRCPSGWHNCRGSGPFIGYCCKQ

Formula C212H328N68O66S6

Absent Amino Acids EMV

Common Amino Acids G

Mass 5077.71

PI 8.31

Basic Residues 6

Acidic Residues 2

Hydrophobic Residues 10

Net Charge +3

Boman Index 2.04

Hydrophobicity -0.435

Aliphatic Index 42.71

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 12865

Absorbance 280nm 253.36

Polar Residues 19

number

1

A

4

R

2

N

2

D

6

C

0

E

1

Q

9

G

1

H

2

I

3

L

1

K

0

M

1

F

3

P

5

S

4

T

2

W

1

Y

0

V

Amino Acid Distribution

A: 1 R: 4 N: 2 D: 2 C: 6 E: 0 Q: 1 G: 9 H: 1 I: 2 L: 3 K: 1 M: 0 F: 1 P: 3 S: 5 T: 4 W: 2 Y: 1 V: 0

Length

48

Formula

C212H328N68O66S6

Mass

5077.71

PI

8.31

Net Charge

+3

Boman Index

2.04

Hydrophobicity

-0.435

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Binds voltage-dependently at site 3 of sodium channels (Nav) and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. Possesses the highest efficacy for the insect sodium channel para/tipE (EC(50)=5.5 nM) (PubMed:12459477), and has effect on Nav1.2/SCN2A (in complex with SCN1B), Nav1.4/SCN4A (in complex with SCN1B) and Nav1.5/SCN5A (in complex with SCN1B) (PubMed:12459477). Also interacts with sodium channels in cardiac cells (PubMed:11704639). Shows lethality to crabs (PubMed:22015268). {ECO:0000269|PubMed:11704639, ECO:0000269|PubMed:12459477, ECO:0000269|PubMed:22015268, ECO:0000269|PubMed:7911468}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Bunodosoma granuliferum (Red warty sea anemone)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Binds voltage-dependently at site 3 of sodium channels (Nav) and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. Possesses the highest efficacy for the insect sodium channel para/tipE (EC(50)=5.5 nM) (PubMed:12459477), and has effect on Nav1.2/SCN2A (in complex with SCN1B), Nav1.4/SCN4A (in complex with SCN1B) and Nav1.5/SCN5A (in complex with SCN1B) (PubMed:12459477).

Protein Name Delta-actitoxin-Bgr2a (Delta-AITX-Bgr2a) (Bg II) (BgII) (Neurotoxin Bg-2)

Venom Taxon Group sea anemone

Protein Family Sea anemone sodium channel inhibitory toxin family, Type I subfamily

Protein Existence Evidence at protein level

Keywords Cardiotoxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Keywords

Cardiotoxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Sea anemone sodium channel inhibitory toxin family, Type I subfamily

Protein_Name

Delta-actitoxin-Bgr2a (Delta-AITX-Bgr2a) (Bg II) (BgII) (Neurotoxin Bg-2)

Venom_Taxon_Group

sea anemone