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Peptide record

TPDB29582

Neurotoxin Toxicity standard
34 amino acids
Basic Information
3D PDB MODEL
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TPDB29582
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 Peptipedia ToxinPred 3.0
standard
No
A 34-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, and ToxinPred 3.0, with an available 3D structural model.
Sequence
DCLGFMRKCIPDNDKCCRPNLVCSRTHKWCKYVF
Physicochemical Analysis
C176H276N52O46S7
AEQ
C
4080.87
8.58
8
3
10
+4
5.05
-0.415
51.47
Mammalian: 1.1 hour Yeast: 3 min E.coli: >10 hour
7365
180.48
11
Residue Composition
number
0
A
3
R
2
N
3
D
6
C
0
E
0
Q
1
G
1
H
1
I
2
L
4
K
1
M
2
F
2
P
1
S
1
T
1
W
1
Y
2
V
Amino Acid Distribution
A: 0 R: 3 N: 2 D: 3 C: 6 E: 0 Q: 0 G: 1 H: 1 I: 1 L: 2 K: 4 M: 1 F: 2 P: 2 S: 1 T: 1 W: 1 Y: 1 V: 2
Chemical Descriptors
34
C176H276N52O46S7
4080.87
8.58
+4
5.05
-0.415
Evidence Records 2 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Potent voltage-gated sodium channel blocker (PubMed:25658507). Potently inhibits the voltage-gated sodium channels Nav1.7/SCN9A (IC(50)=0.58-10 nM) (PubMed:25658507, PubMed:26999206). Also shows a moderate activity on Nav1.1/SCN1A (IC(50)=6 nM), Nav1.2/SCN2A (IC(50)=5-128 nM), Nav1.3/SCN3A (IC(50)=20.3-170 nM), and Nav1.6/SCN8A (IC(50)=17-20.1 nM) (PubMed:25658507, PubMed:26999206). Shows an unclear inhibition of Nav1.4/SCN4A (IC(50)=200 nM to >10 uM), Nav1.5/SCN5A (IC(50)=140 nM to >10 uM) and Nav1.8/SCN10A (IC(50)=68-12200 nM) (PubMed:25658507, PubMed:26999206). Weakly blocks the low voltage-gated calcium channels Cav3.1/CACNA1G (30% inhibition of the peak current at 9.8 nM) (By similarity). shows moderate affinity for lipid bilayers (By similarity). In vivo, when tested on the OD1-induced mouse model of Nav1.7/SCN9A-mediated pain, the toxin is effective when co-administered with OD1, but lacks efficacy when delivered systemically (PubMed:26999206). {ECO:0000250|UniProtKB:P0DJA9, ECO:0000250|UniProtKB:P0DL73, ECO:0000269|PubMed:25658507, ECO:0000269|PubMed:26999206}.

Target

voltage-gated sodium channel (Nav) voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Grammostola porteri (Tarantula spider) (Lasiodora porteri)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Potent voltage-gated sodium channel blocker (PubMed:25658507). Potently inhibits the voltage-gated sodium channels Nav1.7/SCN9A (IC(50)=0.58-10 nM) (PubMed:25658507, PubMed:26999206).
Toxin GTx1-15 (Beta/omega-theraphotoxin-Gr2a) (Beta/omega-TRTX-Gr2a) (Toxin GpTx-1)
spider
Neurotoxin 10 (Hwtx-1) family, 08 (Gtx1-15) subfamily
Evidence at protein level
3D-structure Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:25658507}.
Evidence 2 Activity

Activity

Neurotoxin
FUNCTION: Potent voltage-gated sodium channel blocker (PubMed:24211312). Potently inhibits the voltage-gated sodium channels Nav1.7/SCN9A (IC(50)=0.58-10 nM) (By similarity) (PubMed:24211312). Shows a moderate activity on Nav1.1/SCN1A (IC(50)=6 nM), Nav1.2/SCN2A (IC(50)=5-128 nM), Nav1.3/SCN3A (IC(50)=20.3-170 nM), and Nav1.6/SCN8A (IC(50)=17-20.1 nM) (By similarity) (PubMed:24211312). Shows an unclear inhibition of Nav1.4/SCN4A (IC(50)=200 nM to >10 uM), Nav1.5/SCN5A (IC(50)=140 nM to >10 uM) and Nav1.8/SCN10A (IC(50)=68-12200 nM) (By similarity) (PubMed:24211312). Weakly blocks the low voltage-gated calcium channels Cav3.1/CACNA1G (30% inhibition of the peak current by 9.8 nM of the toxin) (By similarity). It shows moderate affinity for lipid bilayers (By similarity). {ECO:0000250|UniProtKB:P0DJA9, ECO:0000250|UniProtKB:P0DL72, ECO:0000269|PubMed:24211312}.

Target

voltage-gated sodium channel (Nav) voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Paraphysa scrofa (Chilean copper tarantula) (Phrixotrichus auratus)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Potent voltage-gated sodium channel blocker (PubMed:24211312). Potently inhibits the voltage-gated sodium channels Nav1.7/SCN9A (IC(50)=0.58-10 nM) (By similarity) (PubMed:24211312).
Toxin GTx1-15 (Beta/omega-theraphotoxin-Gr2a) (Beta/omega-TRTX-Gr2a) (Toxin GpTx-1)
spider
Neurotoxin 10 (Hwtx-1) family, 08 (Gtx1-15) subfamily
Evidence at protein level
Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:24211312}.
Additional Detail Fields 7 fields
3D-structure Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 10 (Hwtx-1) family, 08 (Gtx1-15) subfamily
Toxin GTx1-15 (Beta/omega-theraphotoxin-Gr2a) (Beta/omega-TRTX-Gr2a) (Toxin GpTx-1)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:25658507}. TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:24211312}.
spider