TPDB

3D PDB MODEL

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TPDB ID TPDB29563

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 33-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, and ToxinPred 3.0, with an available 3D structural model.

Sequence

DCGTIWHYCGTDQSECCEGWKCSRQLCKYVIDW

FASTA

>TPDB29563|Neurotoxin;Toxicity|length=33
DCGTIWHYCGTDQSECCEGWKCSRQLCKYVIDW

Formula C168H243N45O52S6

Absent Amino Acids ANMFP

Common Amino Acids C

Mass 3917.41

PI 4.62

Basic Residues 4

Acidic Residues 5

Hydrophobic Residues 9

Net Charge -2

Boman Index 3.23

Hydrophobicity -0.530

Aliphatic Index 44.24

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: >10 hour

Extinction Coefficient Cystines 19855

Absorbance 280nm 506.84

Polar Residues 14

number

0

A

1

R

0

N

3

D

6

C

2

E

2

Q

3

G

1

H

2

I

1

L

2

K

0

M

0

F

0

P

2

S

2

T

3

W

2

Y

1

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 3 C: 6 E: 2 Q: 2 G: 3 H: 1 I: 2 L: 1 K: 2 M: 0 F: 0 P: 0 S: 2 T: 2 W: 3 Y: 2 V: 1

Length

33

Formula

C168H243N45O52S6

Mass

3917.41

PI

4.62

Net Charge

-2

Boman Index

3.23

Hydrophobicity

-0.530

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Blocks transient outward voltage-gated potassium channels in rat ventricular myocytes (thus prolonging action-potential duration) and rat Kv4.2/KCNA4 channels expressed in Xenopus oocytes. Is also a weak blocker of calcium channels in rat cerebellar granule cells. {ECO:0000269|PubMed:9058605, ECO:0000269|Ref.2}.

Target

Target or Channel voltage-gated potassium channel (Kv) voltage-gated calcium channel (Cav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Heteropoda venatoria (Brown huntsman spider) (Aranea venatoria)

Neurotoxin Type Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Mechanism

Blocks transient outward voltage-gated potassium channels in rat ventricular myocytes (thus prolonging action-potential duration) and rat Kv4.2/KCNA4 channels expressed in Xenopus oocytes. Is also a weak blocker of calcium channels in rat cerebellar granule cells..

Protein Name Kappa-sparatoxin-Hv1a (Kappa-SPRTX-Hv1a) (Heteropodatoxin-1) (HpTX1) (Toxin AU3/KJ5)

Venom Taxon Group spider

Protein Family Neurotoxin 10 (Hwtx-1) family, 21 (HpTx1) subfamily

Protein Existence Evidence at protein level

Keywords Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:9058605, ECO:0000305|Ref.2}.

Keywords

Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin

Neurotoxin_Type

Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 10 (Hwtx-1) family, 21 (HpTx1) subfamily

Protein_Name

Kappa-sparatoxin-Hv1a (Kappa-SPRTX-Hv1a) (Heteropodatoxin-1) (HpTX1) (Toxin AU3/KJ5)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:9058605, ECO:0000305|Ref.2}.

Venom_Taxon_Group

spider