TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB28875

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 48-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2 and Peptipedia, with an available 3D structural model.

Sequence

CGDINAACKEDCDCCGYTTACDCYWSSSCKCREAAIVIYTAPKKKLTC

FASTA

>TPDB28875|Neurotoxin;Toxicity|length=48
CGDINAACKEDCDCCGYTTACDCYWSSSCKCREAAIVIYTAPKKKLTC

Formula C215H340N58O72S10

Absent Amino Acids QHMF

Common Amino Acids C

Mass 5210.01

PI 6.07

Basic Residues 6

Acidic Residues 6

Hydrophobic Residues 15

Net Charge -0

Boman Index 7.05

Hydrophobicity -0.075

Aliphatic Index 51.04

Half Life

Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 10595

Absorbance 280nm 203.36

Polar Residues 21

number

6

A

1

R

1

N

4

D

10

C

2

E

0

Q

2

G

0

H

3

I

1

L

5

K

0

M

0

F

1

P

3

S

4

T

1

W

3

Y

1

V

Amino Acid Distribution

A: 6 R: 1 N: 1 D: 4 C: 10 E: 2 Q: 0 G: 2 H: 0 I: 3 L: 1 K: 5 M: 0 F: 0 P: 1 S: 3 T: 4 W: 1 Y: 3 V: 1

Length

48

Formula

C215H340N58O72S10

Mass

5210.01

PI

6.07

Net Charge

-0

Boman Index

7.05

Hydrophobicity

-0.075

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Insecticidal neurotoxin that reversibly inhibits the N-methyl-D-aspartate (NMDA)-subtype of ionotropic glutamate receptor (GRIN) and inhibits inactivation of insect sodium channels (Nav) (By similarity). Inhibits glutamate uptake in rat brain synaptosomes (PubMed:14757211). In vivo, induces immediate excitatory effects when injected intrathoracically in houseflies and cockroaches (PubMed:14757211). {ECO:0000250|UniProtKB:P59367, ECO:0000269|PubMed:14757211}.

Target

Target or Channel voltage-gated sodium channel (Nav) glutamate receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Voltage-gated sodium channel impairing toxin

Mechanism

Insecticidal neurotoxin that reversibly inhibits the N-methyl-D-aspartate (NMDA)-subtype of ionotropic glutamate receptor (GRIN) and inhibits inactivation of insect sodium channels (Nav) (By similarity). Inhibits glutamate uptake in rat brain synaptosomes (PubMed:14757211).

Protein Name Delta-ctenitoxin-Pn1b (Delta-CNTX-Pn1b) (Neurotoxin PnTx4-3)

Venom Taxon Group spider

Protein Family Neurotoxin 03 (Tx2) family, 05 subfamily

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Knottin Neurotoxin Postsynaptic neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:14757211, ECO:0000305|PubMed:16278100}.

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Knottin Neurotoxin Postsynaptic neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 03 (Tx2) family, 05 subfamily

Protein_Name

Delta-ctenitoxin-Pn1b (Delta-CNTX-Pn1b) (Neurotoxin PnTx4-3)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:14757211, ECO:0000305|PubMed:16278100}.

Venom_Taxon_Group

spider