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Peptide record

TPDB27863

Neurotoxin Toxicity standard
42 amino acids
Basic Information
3D PDB MODEL
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TPDB27863
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 pep-lab Peptipedia
standard
No
A 42-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, pep-lab, and Peptipedia, with an available 3D structural model.
Sequence
GTTCYCGKTIGIYWFGTKTCPSNRGYTGSCGYFLGICCYPVD
Physicochemical Analysis
C203H295N49O59S6
AEQHM
G
4558.23
8.01
3
1
13
+2
-5.15
0.076
44.05
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
13325
292.33
20
Residue Composition
number
0
A
1
R
1
N
1
D
6
C
0
E
0
Q
8
G
0
H
3
I
1
L
2
K
0
M
2
F
2
P
2
S
6
T
1
W
5
Y
1
V
Amino Acid Distribution
A: 0 R: 1 N: 1 D: 1 C: 6 E: 0 Q: 0 G: 8 H: 0 I: 3 L: 1 K: 2 M: 0 F: 2 P: 2 S: 2 T: 6 W: 1 Y: 5 V: 1
Chemical Descriptors
42
C203H295N49O59S6
4558.23
8.01
+2
-5.15
0.076
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Peptide with both antimicrobial and neurotoxin activities. This toxin acts both on ERG potassium channels and sodium channels (PubMed:12815161, PubMed:16497878, PubMed:17473056, PubMed:22972919). It potently and reversibly inhibits human Kv11.1/KCNH2/ERG1 (IC(50)=34 nM) (PubMed:12815161, PubMed:16497878, PubMed:17473056), rat Kv11.1/KCNH2/ERG1 (PubMed:16497878) and Kv11.3/KCNH7/ERG3 (PubMed:17473056) voltage-gated potassium channels in a similar potency. It acts as a gating-modifier toxin that shifts the voltage-dependence of ERG activation in the positive direction and suppresses its current amplitudes elicited by strong depolarizing pulses (PubMed:12815161, PubMed:17473056). On sodium channels, it blocks Nav1.2/SCN2A (EC(50)=31 nM), Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A, Nav1.6/SCN8A, Nav1.8/SCN10A (EC(50)=92 nM) (PubMed:22972919). It may act by binding at site 1 or close by, only when the pore is in an open configuration (PubMed:22972919). Shows antibacterial activity against the Gram-negative bacterium S.typhimurium, but not on the bacteria B.subtilis, S.aureus, and P.aeruginosa (PubMed:28796463). In vivo, this toxin does not induce neurotoxic symptoms when injected into mice (PubMed:12815161). {ECO:0000269|PubMed:12815161, ECO:0000269|PubMed:16497878, ECO:0000269|PubMed:17473056, ECO:0000269|PubMed:22972919}.

Target

voltage-gated sodium channel (Nav) voltage-gated potassium channel (Kv)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Anthopleura elegantissima (Green aggregating anemone) (Actinia elegantissima)
Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Peptide with both antimicrobial and neurotoxin activities. This toxin acts both on ERG potassium channels and sodium channels (PubMed:12815161, PubMed:16497878, PubMed:17473056, PubMed:22972919).
Kappa-actitoxin-Ael2a (Kappa-AITX-Ael2a) (Toxin APETx1)
sea anemone
Sea anemone type 3 (BDS) potassium channel toxin family
Evidence at protein level
3D-structure Antibiotic Antimicrobial Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Additional Detail Fields 6 fields
3D-structure Antibiotic Antimicrobial Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Sea anemone type 3 (BDS) potassium channel toxin family
Kappa-actitoxin-Ael2a (Kappa-AITX-Ael2a) (Toxin APETx1)
sea anemone