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Peptide record

TPDB27862

Neurotoxin Toxicity standard
42 amino acids
Basic Information
3D PDB MODEL
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TPDB27862
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 pep-lab Peptipedia
standard
No
A 42-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, pep-lab, and Peptipedia, with an available 3D structural model.
Sequence
GTACSCGNSKGIYWFYRPSCPTDRGYTGSCRYFLGTCCTPAD
Physicochemical Analysis
C196H286N54O61S6
EQHMV
CG
4567.11
8.01
4
2
11
+2
-1.59
-0.367
23.33
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
11835
259.14
20
Residue Composition
number
2
A
3
R
1
N
2
D
6
C
0
E
0
Q
6
G
0
H
1
I
1
L
1
K
0
M
2
F
3
P
4
S
5
T
1
W
4
Y
0
V
Amino Acid Distribution
A: 2 R: 3 N: 1 D: 2 C: 6 E: 0 Q: 0 G: 6 H: 0 I: 1 L: 1 K: 1 M: 0 F: 2 P: 3 S: 4 T: 5 W: 1 Y: 4 V: 0
Chemical Descriptors
42
C196H286N54O61S6
4567.11
8.01
+2
-1.59
-0.367
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: This toxin potently blocks acid-sensing ion channel ASIC3 homotrimers and heterotrimers containing ASIC3 (composed with isoforms of ASIC1 and ASIC2) (PubMed:15044953). It also weakly inhibits potassium channels, and sodium channels (PubMed:15044953, PubMed:21943094, PubMed:22972919, PubMed:25337890). On homomeric ASIC3, this protein shows IC(50)=57-87 nM on rat, 37.3 nM on mouse and 175 nM on human channels (PubMed:15044953, PubMed:18923424, PubMed:19306891, PubMed:20813121, PubMed:21943094, PubMed:22851922, PubMed:22851929). The blockade is rapid and reversible (PubMed:15044953). On heterotrimeric forms, the toxin is less potent (IC(50)=117 nM on rat ASIC2b-ASIC3 channel, 900 nM on rat ASIC1b-ASIC3, and 2 uM on rat ASIC1a-ASIC3) (PubMed:15044953). It weakly inhibits Kv3.4/KCNC4 potassium channels (3 uM of the toxin inhibits 38% of Kv3.4 current) (PubMed:15044953). It reversibly and voltage-dependently inhibits hKv11.1/KCNH2/ERG1 potassium channels (IC(50)=1.21 uM), inhibiting both peak and tail currents without action on channel inactivation (PubMed:25337890). It weakly inhibits rNav1.2/SCN2A (EC(50)=114 nM), rNav1.6/SCN8A current (17% at 1 uM of the toxin) and Nav1.8/SCN10A (IC(50)=6.6 uM on human channels expressed in oocytes, EC(50)=55 nM on rat channels expressed in oocytes, and 2.6 uM on rat channels in DRG neurons) (PubMed:21943094, PubMed:22972919). It may act on sodium channels by binding at site 1 or close by, when the pore is in an open configuration (PubMed:22972919). In vivo, central injection does not induce neurotoxin symptoms in mice even after 24 hours (PubMed:15044953). However, it abolishes acid-induced pain in rats (PubMed:18923424). {ECO:0000269|PubMed:15044953, ECO:0000269|PubMed:18923424, ECO:0000269|PubMed:19306891, ECO:0000269|PubMed:20813121, ECO:0000269|PubMed:21943094, ECO:0000269|PubMed:22851922, ECO:0000269|PubMed:22851929, ECO:0000269|PubMed:22972919, ECO:0000269|PubMed:25337890}.

Target

voltage-gated sodium channel (Nav) voltage-gated potassium channel (Kv) proton-gated sodium channel / ASIC

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Anthopleura elegantissima (Green aggregating anemone) (Actinia elegantissima)
Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Proton-gated sodium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
This toxin potently blocks acid-sensing ion channel ASIC3 homotrimers and heterotrimers containing ASIC3 (composed with isoforms of ASIC1 and ASIC2) (PubMed:15044953). It also weakly inhibits potassium channels, and sodium channels (PubMed:15044953, PubMed:21943094, PubMed:22972919, PubMed:25337890).
Pi-actitoxin-Ael2b (Pi-AITX-Ael2b) (Toxin APETx2)
sea anemone
Sea anemone type 3 (BDS) potassium channel toxin family
Evidence at protein level
3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Pharmaceutical Potassium channel impairing toxin Proton-gated sodium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Additional Detail Fields 6 fields
3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Pharmaceutical Potassium channel impairing toxin Proton-gated sodium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Proton-gated sodium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Sea anemone type 3 (BDS) potassium channel toxin family
Pi-actitoxin-Ael2b (Pi-AITX-Ael2b) (Toxin APETx2)
sea anemone