TPDB

3D PDB MODEL

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TPDB ID TPDB27841

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 pep-lab Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 38-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, pep-lab, and Peptipedia, with an available 3D structural model.

Sequence

GVPINVSCTGSPQCIKPCKDAGMRFGKCMNRKCHCTPK

FASTA

>TPDB27841|Neurotoxin;Toxicity|length=38
GVPINVSCTGSPQCIKPCKDAGMRFGKCMNRKCHCTPK

Formula C169H284N54O48S8

Absent Amino Acids ELWY

Common Amino Acids C

Mass 4096.93

PI 9.30

Basic Residues 8

Acidic Residues 1

Hydrophobic Residues 8

Net Charge +6

Boman Index 8.88

Hydrophobicity -0.418

Aliphatic Index 38.42

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 375

Absorbance 280nm 9.15

Polar Residues 13

number

1

A

2

R

2

N

1

D

6

C

0

E

1

Q

4

G

1

H

2

I

0

L

5

K

2

M

1

F

4

P

2

S

2

T

0

W

0

Y

2

V

Amino Acid Distribution

A: 1 R: 2 N: 2 D: 1 C: 6 E: 0 Q: 1 G: 4 H: 1 I: 2 L: 0 K: 5 M: 2 F: 1 P: 4 S: 2 T: 2 W: 0 Y: 0 V: 2

Length

38

Formula

C169H284N54O48S8

Mass

4096.93

PI

9.30

Net Charge

+6

Boman Index

8.88

Hydrophobicity

-0.418

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Potent inhibitor of the Shaker potassium channels and its mammalian homologs (Kv1.1/KCNA1, Kv1.3/KCNA3, Kv1.6/KCNA6) (Ki<1 nM for all channels) (PubMed:20007782, PubMed:8204618). Also blocks Kv1.2/KCNA2 (IC(50)=26.8 nM) (PubMed:20007782, PubMed:8204618). It also shows a weak interaction with nicotinic acetylcholine receptors (nAChR), suggesting it may weakly inhibit it (PubMed:31276191). {ECO:0000269|PubMed:20007782, ECO:0000269|PubMed:8204618}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor voltage-gated potassium channel (Kv)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Leiurus hebraeus (Hebrew deathstalker scorpion) (Leiurus quinquestriatus hebraeus)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Mechanism

Potent inhibitor of the Shaker potassium channels and its mammalian homologs (Kv1.1/KCNA1, Kv1.3/KCNA3, Kv1.6/KCNA6) (Ki<1 nM for all channels) (PubMed:20007782, PubMed:8204618). Also blocks Kv1.2/KCNA2 (IC(50)=26.8 nM) (PubMed:20007782, PubMed:8204618).

Protein Name Potassium channel toxin alpha-KTx 3.2 (Agitoxin-2) (AgTx-2) (AgTx2)

Venom Taxon Group scorpion

Protein Family Short scorpion toxin superfamily, Potassium channel inhibitor family, Alpha-KTx 03 subfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:8204618}.

Keywords

3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Short scorpion toxin superfamily, Potassium channel inhibitor family, Alpha-KTx 03 subfamily

Protein_Name

Potassium channel toxin alpha-KTx 3.2 (Agitoxin-2) (AgTx-2) (AgTx2)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:8204618}.

Venom_Taxon_Group

scorpion