Back to search

Peptide record

TPDB27823

Neurotoxin Toxicity standard
37 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB27823
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 Peptipedia pep-lab
standard
No
A 37-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, and pep-lab, with an available 3D structural model.
Sequence
CNSKGTPCTNADECCGGKCAYNVWNCIGGGCSKTCGY
Physicochemical Analysis
C150H231N45O53S8
RQHLMF
C
3769.23
7.48
3
2
7
+1
2.87
-0.324
23.78
Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour
8980
238.24
19
Residue Composition
number
2
A
0
R
4
N
1
D
8
C
1
E
0
Q
7
G
0
H
1
I
0
L
3
K
0
M
0
F
1
P
2
S
3
T
1
W
2
Y
1
V
Amino Acid Distribution
A: 2 R: 0 N: 4 D: 1 C: 8 E: 1 Q: 0 G: 7 H: 0 I: 1 L: 0 K: 3 M: 0 F: 0 P: 1 S: 2 T: 3 W: 1 Y: 2 V: 1
Chemical Descriptors
37
C150H231N45O53S8
3769.23
7.48
+1
2.87
-0.324
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: The recombinant mu-cyrtautoxin-As1a potently and voltage-independently blocks voltage-gated sodium channels (Nav) of insects. It acts by pluging the outer vestibule of the channel. It acts in combination with a weak (30%) voltage-independent block of insect voltage-gated calcium (Cav) channels (low-voltage and high-voltage channels). Tested on DUM neurons, it inhibits sodium currents with an IC(50) of 540 nM (and a Hill coefficient >1, reflecting an incomplete block at higher concentrations). In vivo, it induces flaccid paralysis in adult Australian sheep blowfly Lucilia cuprina (PubMed:23473802). It is both paralytic and lethal, when injected into lepidopteran larvae. It is a slower acting toxin, being lethal at 24 hours, but not paralytic at 1 hour post-injection (PubMed:1440641). {ECO:0000269|PubMed:1440641, ECO:0000269|PubMed:23473802}.

Target

voltage-gated sodium channel (Nav) voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Apomastus schlingeri (Trap-door spider) (Aptostichus schlingeri)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
The recombinant mu-cyrtautoxin-As1a potently and voltage-independently blocks voltage-gated sodium channels (Nav) of insects. It acts by pluging the outer vestibule of the channel.
Mu-cyrtautoxin-As1a (Mu-CUTX-As1a) (Aptotoxin III) (Aps III) (Aptotoxin-3) (Aps-3) (Paralytic peptide III) (PP III)
spider
Neurotoxin 13 (insecticidal toxin ABC) family, 01 (Aps III) subfamily
Evidence at protein level
3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:1440641}.
Additional Detail Fields 7 fields
3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 13 (insecticidal toxin ABC) family, 01 (Aps III) subfamily
Mu-cyrtautoxin-As1a (Mu-CUTX-As1a) (Aptotoxin III) (Aps III) (Aptotoxin-3) (Aps-3) (Paralytic peptide III) (PP III)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:1440641}.
spider