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Peptide record

TPDB27797

Neurotoxin Toxicity standard
35 amino acids
Basic Information
3D PDB MODEL
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TPDB27797
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 pep-lab Peptipedia ToxinPred 3.0
standard
No
A 35-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, pep-lab, Peptipedia, and other sources, with an available 3D structural model.
Sequence
GCIAKNKECAWFSGEWCCGALSCKYSIKRNLKICV
Physicochemical Analysis
C171H271N47O46S6
DQHMPT
C
3913.68
8.58
6
2
13
+4
1.36
0.109
72.57
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
12865
328.72
12
Residue Composition
number
3
A
1
R
2
N
0
D
6
C
2
E
0
Q
3
G
0
H
3
I
2
L
5
K
0
M
1
F
0
P
3
S
0
T
2
W
1
Y
1
V
Amino Acid Distribution
A: 3 R: 1 N: 2 D: 0 C: 6 E: 2 Q: 0 G: 3 H: 0 I: 3 L: 2 K: 5 M: 0 F: 1 P: 0 S: 3 T: 0 W: 2 Y: 1 V: 1
Chemical Descriptors
35
C171H271N47O46S6
3913.68
8.58
+4
1.36
0.109
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Gating-modifier toxin that inhibits mammalian and insect voltage-gated sodium channels. It shifts the voltage dependence of channel activation to more positive voltages. It shows potent activity on Nav1.4/SCN4A (IC(50)=103 nM), Nav1.5/SCN5A (IC(50)=268 nM) and Para/DmNav1 (IC(50)=555 nM) and lower activities on Nav1.2/SCN2A (IC(50)=1447 nM) and Nav1.6/SCN8A (IC(50)=3504 nM) (PubMed:25352595). In addition, at a concentration of 1 uM, the toxin inhibits 90-100% of sodium current through Nav1.2/SCN2A, Nav1.4/SCN4A, Nav1.5/SCN5A, Nav1.6/SCN8A and Para/DmNav1 channels, when the voltage of maximal activation of the channel in control conditions is applied (Ref.1). It binds to the S3-S4 helix-loop-helix motif in the voltage-sensing domain of repeat 1 (shown on hNav1.4/SCN4A) (PubMed:29636418). The toxin is amphiphilic and binds to both neutral and negatively charged lipid vesicles with high affinity (PubMed:25352595, PubMed:29636418). The hydrophobic face lies on the opposite side to the hydrophobic faces of classical gating modifiers (PubMed:25352595). {ECO:0000269|PubMed:25352595, ECO:0000269|Ref.1}.

Target

voltage-gated sodium channel (Nav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Heriaeus mellotteei (Crab spider) (Heriaeus oblongus)
Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin
Gating-modifier toxin that inhibits mammalian and insect voltage-gated sodium channels. It shifts the voltage dependence of channel activation to more positive voltages.
Mu-thomitoxin-Hme1c (Mu-TMTX-Hme1c) (Neurotoxin Hm-3)
spider
Neurotoxin 07 (Beta/delta-agtx) family
Evidence at protein level
3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
Additional Detail Fields 7 fields
3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 07 (Beta/delta-agtx) family
Mu-thomitoxin-Hme1c (Mu-TMTX-Hme1c) (Neurotoxin Hm-3)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
spider