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Peptide record

TPDB27795

Neurotoxin Toxicity standard
35 amino acids
Basic Information
3D PDB MODEL
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TPDB27795
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 Peptipedia pep-lab ToxinPred 3.0
standard
No
A 35-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, pep-lab, and other sources, with an available 3D structural model.
Sequence
ECRYWLGGCSAGQTCCKHLVCSRRHGWCVWDGTFS
Physicochemical Analysis
C171H251N53O47S6
NIMP
C
3993.56
8.02
6
2
10
+2
-0.32
-0.243
41.71
Mammalian: 1 hour Yeast: 30 min E.coli: >10 hour
18365
459.87
13
Residue Composition
number
1
A
3
R
0
N
1
D
6
C
1
E
1
Q
5
G
2
H
0
I
2
L
1
K
0
M
1
F
0
P
3
S
2
T
3
W
1
Y
2
V
Amino Acid Distribution
A: 1 R: 3 N: 0 D: 1 C: 6 E: 1 Q: 1 G: 5 H: 2 I: 0 L: 2 K: 1 M: 0 F: 1 P: 0 S: 3 T: 2 W: 3 Y: 1 V: 2
Chemical Descriptors
35
C171H251N53O47S6
3993.56
8.02
+2
-0.32
-0.243
Evidence Records 2 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Ion channel impairing toxin that inhibits voltage-gated calcium channel Cav3.1/CACNA1G (IC(50)=53 nM), voltage-gated potassium channels Kv2.1/KCNB1 (IC(50)=411 nM), all sodium channels tested (Nav1.2/SCN2A (IC(50)=60-104 nM), Nav1.5/SCN5A (IC(50)=76-358 nM), Nav1.6/SCN8A (IC(50)=21-133 nM), Nav1.7/SCN9A (IC(50)=51-95 nM), and Nav1.8/SCN10A) as well as the nociceptor cation channel TRPA1 (IC(50)=389 nM) (By similarity) (PubMed:32511987). Acts as a potent and selective blocker of voltage-gated calcium channel Cav3.1/CACNA1G, but not of Cav3.2/CACNA1H, and Cav3.3/CACNA1I (By similarity). On Nav1.7/SCN9A, primarily interacts with the DII and DIV voltage-sensor domains (PubMed:32511987). Also acts as an inhibitor of nociceptor cation channel TRPA1 (IC(50)~389 nM) by binding to the S1-S4 gating domain of TRPA1 (By similarity). It shows moderate affinity for lipid bilayers (By similarity). {ECO:0000250|UniProtKB:P83480, ECO:0000269|PubMed:32511987}.

Target

voltage-gated sodium channel (Nav) voltage-gated potassium channel (Kv) voltage-gated calcium channel (Cav) TRP channel

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Bumba pulcherrimaklaasi (Tarantula spider) (Euathlus pulcherrimaklaasi)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Ion channel impairing toxin that inhibits voltage-gated calcium channel Cav3.1/CACNA1G (IC(50)=53 nM), voltage-gated potassium channels Kv2.1/KCNB1 (IC(50)=411 nM), all sodium channels tested (Nav1.2/SCN2A (IC(50)=60-104 nM), Nav1.5/SCN5A (IC(50)=76-358 nM), Nav1.6/SCN8A (IC(50)=21-133 nM), Nav1.7/SCN9A (IC(50)=51-95 nM), and Nav1.8/SCN10A) as well as the nociceptor cation channel TRPA1 (IC(50)=389 nM) (By similarity) (PubMed:32511987). Acts as a potent and selective blocker of voltage-gated calcium channel Cav3.1/CACNA1G, but not of Cav3.2/CACNA1H, and Cav3.3/CACNA1I (By similarity).
Beta/omega-theraphotoxin-Bp1a (Beta/omega-TRTX-Bp1a) (Protoxin-I analog) (ProTx-I analog)
spider
Neurotoxin 10 (Hwtx-1) family, 54 (ProTx-1) subfamily
Evidence at protein level
Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Potassium channel impairing toxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:32511987}.
Evidence 2 Activity

Activity

Neurotoxin
FUNCTION: Ion channel impairing toxin that inhibits voltage-gated calcium channel Cav3.1/CACNA1G (IC(50)=53 nM), voltage-gated potassium channels Kv2.1/KCNB1 (IC(50)=411 nM), all sodium channels tested (Nav1.2/SCN2A (IC(50)=60-104 nM), Nav1.5/SCN5A (IC(50)=76-358 nM), Nav1.6/SCN8A (IC(50)=21-133 nM), Nav1.7/SCN9A (IC(50)=51-95 nM), and Nav1.8/SCN10A) and the nociceptor cation channel TRPA1 (IC(50)=389 nM) (PubMed:12475222, PubMed:17087985, PubMed:20351484, PubMed:24530065, PubMed:24886690, PubMed:29703751, PubMed:31234412). Acts as a potent and selective blocker of voltage-gated calcium channel Cav3.1/CACNA1G, but not of Cav3.2/CACNA1H, and Cav3.3/CACNA1I (PubMed:20351484, PubMed:24886690). On Nav1.7/SCN9A, primarily interacts with the DII and DIV voltage-sensor domains (PubMed:32511987). Also acts as an inhibitor of nociceptor cation channel TRPA1 (IC(50)~389 nM) by binding to the S1-S4 gating domain of TRPA1 (PubMed:24530065). It shows moderate affinity for lipid bilayers (PubMed:29703751). {ECO:0000269|PubMed:12475222, ECO:0000269|PubMed:17087985, ECO:0000269|PubMed:20351484, ECO:0000269|PubMed:24530065, ECO:0000269|PubMed:24886690, ECO:0000269|PubMed:29703751, ECO:0000269|PubMed:31234412}.

Target

voltage-gated sodium channel (Nav) voltage-gated potassium channel (Kv) voltage-gated calcium channel (Cav) TRP channel

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Thrixopelma pruriens (Peruvian green velvet tarantula)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Ion channel impairing toxin that inhibits voltage-gated calcium channel Cav3.1/CACNA1G (IC(50)=53 nM), voltage-gated potassium channels Kv2.1/KCNB1 (IC(50)=411 nM), all sodium channels tested (Nav1.2/SCN2A (IC(50)=60-104 nM), Nav1.5/SCN5A (IC(50)=76-358 nM), Nav1.6/SCN8A (IC(50)=21-133 nM), Nav1.7/SCN9A (IC(50)=51-95 nM), and Nav1.8/SCN10A) and the nociceptor cation channel TRPA1 (IC(50)=389 nM) (PubMed:12475222, PubMed:17087985, PubMed:20351484, PubMed:24530065, PubMed:24886690, PubMed:29703751, PubMed:31234412). Acts as a potent and selective blocker of voltage-gated calcium channel Cav3.1/C
Beta/omega-theraphotoxin-Tp1a (Beta/omega-TRTX-Tp1a) (Protoxin-1) (ProTx-1) (ProTx1) (Protoxin-I) (ProTx-I)
spider
Neurotoxin 10 (Hwtx-1) family, 54 (ProTx-1) subfamily
Evidence at protein level
3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Potassium channel impairing toxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:12475222}.
Additional Detail Fields 7 fields
Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Potassium channel impairing toxin Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin 3D-structure
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 10 (Hwtx-1) family, 54 (ProTx-1) subfamily
Beta/omega-theraphotoxin-Bp1a (Beta/omega-TRTX-Bp1a) (Protoxin-I analog) (ProTx-I analog) Beta/omega-theraphotoxin-Tp1a (Beta/omega-TRTX-Tp1a) (Protoxin-1) (ProTx-1) (ProTx1) (Protoxin-I) (ProTx-I)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:32511987}. TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:12475222}.
spider