TPDB

3D PDB MODEL

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TPDB ID TPDB27787

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia pep-lab ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 34-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, pep-lab, and other sources, with an available 3D structural model.

Sequence

TCRYLFGGCKTTADCCKHLACRSDGKYCAWDGTF

FASTA

>TPDB27787|Neurotoxin;Toxicity|length=34
TCRYLFGGCKTTADCCKHLACRSDGKYCAWDGTF

Formula C161H242N46O48S6

Absent Amino Acids NEQIMPV

Common Amino Acids C

Mass 3782.33

PI 8.02

Basic Residues 6

Acidic Residues 3

Hydrophobic Residues 10

Net Charge +2

Boman Index 0.91

Hydrophobicity -0.279

Aliphatic Index 31.76

Half Life

Mammalian: 7.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 8855

Absorbance 280nm 234.11

Polar Residues 13

number

3

A

2

R

0

N

3

D

6

C

0

E

0

Q

4

G

1

H

0

I

2

L

3

K

0

M

2

F

0

P

1

S

4

T

1

W

2

Y

0

V

Amino Acid Distribution

A: 3 R: 2 N: 0 D: 3 C: 6 E: 0 Q: 0 G: 4 H: 1 I: 0 L: 2 K: 3 M: 0 F: 2 P: 0 S: 1 T: 4 W: 1 Y: 2 V: 0

Length

34

Formula

C161H242N46O48S6

Mass

3782.33

PI

8.02

Net Charge

+2

Boman Index

0.91

Hydrophobicity

-0.279

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Reversibly inhibits potassium currents in oocytes expressing Kv2.1/KCNB1 channels (Kd=2.7 uM) (PubMed:15051809). Acts by shifting activation of the channel to more depolarized voltages. The toxin may bind to the S3b-S4 helices of the voltage sensor paddle. One, two, three or four toxin molecules may bind the Kv2.1/KCNB1 channel. It shows low to moderate affinity for lipid bilayers (PubMed:29703751). It partitions into the bilayer membrane, where it stabilizes at the water/membrane interface (PubMed:17071657, PubMed:20643084). {ECO:0000269|PubMed:10504388, ECO:0000269|PubMed:14744131, ECO:0000269|PubMed:15051809, ECO:0000269|PubMed:17071657, ECO:0000269|PubMed:20643084, ECO:0000269|PubMed:29703751}.

Target

Target or Channel voltage-gated potassium channel (Kv)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Stromatopelma calceatum griseipes (Feather leg baboon tarantula) (Scodra griseipes)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Mechanism

Reversibly inhibits potassium currents in oocytes expressing Kv2.1/KCNB1 channels (Kd=2.7 uM) (PubMed:15051809). Acts by shifting activation of the channel to more depolarized voltages.

Protein Name Kappa-theraphotoxin-Scg1a (Kappa-TRTX-Scg1a) (SGTx1) (SGTx) (SgTx-I)

Venom Taxon Group spider

Protein Family Neurotoxin 10 (Hwtx-1) family, 09 (HaTx) subfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10504388}.

Keywords

3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 10 (Hwtx-1) family, 09 (HaTx) subfamily

Protein_Name

Kappa-theraphotoxin-Scg1a (Kappa-TRTX-Scg1a) (SGTx1) (SGTx) (SgTx-I)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10504388}.

Venom_Taxon_Group

spider