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Peptide record

TPDB27776

Cell-penetrating Peptides Neurotoxin Toxicity standard
33 amino acids
Basic Information
3D PDB MODEL
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TPDB27776
Cell-penetrating Peptides Neurotoxin Toxicity
Toxicity and safety peptides Delivery and barrier-penetrating peptides
Peptipedia NTxPred2 pep-lab ToxinPred 3.0
standard
No
A 33-aa standard natural multi-activity (Cell-penetrating Peptides, Neurotoxin, and Toxicity) peptide sequence curated from Peptipedia, NTxPred2, pep-lab, and other sources, with an available 3D structural model.
Sequence
GDCLPHLKLCKENKDCCSKKCKRRGTNIEKRCR
Physicochemical Analysis
C156H276N56O46S6
AQMFWYV
K
3864.62
9.62
12
4
4
+7
15.78
-1.288
47.27
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
375
9.70
10
Residue Composition
number
0
A
4
R
2
N
2
D
6
C
2
E
0
Q
2
G
1
H
1
I
3
L
7
K
0
M
0
F
1
P
1
S
1
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 4 N: 2 D: 2 C: 6 E: 2 Q: 0 G: 2 H: 1 I: 1 L: 3 K: 7 M: 0 F: 0 P: 1 S: 1 T: 1 W: 0 Y: 0 V: 0
Chemical Descriptors
33
C156H276N56O46S6
3864.62
9.62
+7
15.78
-1.288
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: This toxin stabilizes ryanodine receptor 1 (RyR1) opening in a long-lasting subconductance state (48%-60% of the full conductance state) (PubMed:10713267, PubMed:12869557, PubMed:27114612). Furthermore, it triggers calcium release from sarcoplasmic vesicles (6.6 nM are enough to induce a sharp release, and 60% of the total calcium is released after toxin (100 nM) addition) probably by acting as a cell-penetrating peptide (CPP) (PubMed:27114612). In addition, it has been shown to dose-dependently stimulate ryanodine binding to RyR1 (EC(50)=12.5-26.4 nM) (PubMed:12869557, PubMed:17291197, PubMed:17888395, PubMed:27114612). It also augments the bell-shaped calcium-[3H]ryanodine binding curve that is maximal at about 10 uM calcium concentration (PubMed:27114612). It binds a different site as ryanodine (PubMed:10713267). It acts synergistically with caffeine (By similarity). In vivo, intracerebroventricular injection into mice causes death (PubMed:10713267). {ECO:0000250|UniProtKB:A0A1L4BJ42, ECO:0000250|UniProtKB:B8QG00, ECO:0000250|UniProtKB:P59868, ECO:0000269|PubMed:10713267, ECO:0000269|PubMed:12869557, ECO:0000269|PubMed:17291197, ECO:0000269|PubMed:17888395, ECO:0000269|PubMed:27114612}.

Target

voltage-gated calcium channel (Cav) ryanodine receptor

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Scorpio palmatus (Israeli golden scorpion) (Scorpio maurus palmatus)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Ryanodine-sensitive calcium-release channel impairing toxin
This toxin stabilizes ryanodine receptor 1 (RyR1) opening in a long-lasting subconductance state (48%-60% of the full conductance state) (PubMed:10713267, PubMed:12869557, PubMed:27114612). Furthermore, it triggers calcium release from sarcoplasmic vesicles (6.6 nM are enough to induce a sharp release, and 60% of the total calcium is released after toxin (100 nM) addition) probably by acting as a cell-penetrating peptide (CPP) (PubMed:27114612).
Maurocalcin (MCa) (Maurocalcine)
scorpion
Scorpion calcin family
Evidence at protein level
3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Ryanodine-sensitive calcium-release channel impairing toxin Secreted Toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10713267}.
Additional Detail Fields 7 fields
3D-structure Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Ryanodine-sensitive calcium-release channel impairing toxin Secreted Toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Ryanodine-sensitive calcium-release channel impairing toxin
Evidence at protein level
Scorpion calcin family
Maurocalcin (MCa) (Maurocalcine)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10713267}.
scorpion