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Peptide record

TPDB27772

Neurotoxin Toxicity standard
33 amino acids
Basic Information
3D PDB MODEL
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TPDB27772
Neurotoxin Toxicity
Toxicity and safety peptides
NTxPred2 Peptipedia pep-lab ToxinPred 3.0
standard
No
A 33-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, pep-lab, and other sources, with an available 3D structural model.
Sequence
DCLGWFKSCDPKNDKCCKNYTCSRRDRWCKYDL
Physicochemical Analysis
C172H261N51O51S6
AEQHIMV
C
4051.64
8.30
8
5
7
+3
5.96
-1.236
23.64
Mammalian: 1.1 hour Yeast: 3 min E.coli: >10 hour
14355
354.30
13
Residue Composition
number
0
A
3
R
2
N
5
D
6
C
0
E
0
Q
1
G
0
H
0
I
2
L
5
K
0
M
1
F
1
P
2
S
1
T
2
W
2
Y
0
V
Amino Acid Distribution
A: 0 R: 3 N: 2 D: 5 C: 6 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 2 K: 5 M: 0 F: 1 P: 1 S: 2 T: 1 W: 2 Y: 2 V: 0
Chemical Descriptors
33
C172H261N51O51S6
4051.64
8.30
+3
5.96
-1.236
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Inhibits many voltage-gated sodium channels and one voltage-gated calcium channel (Cav2.2/CACNA1B (IC(50)=400 nM), Nav1.2/SCN2A (IC(50)=3-70 nM), Nav1.1/SCN1A (IC(50)=523-1060 nM), Nav1.7/SCN9A (IC(50)=129.1-5120 nM), Nav1.4/SCN4A (IC(50)=263-888 nM or >10 uM) and Nav1.5/SCN5A (IC(50)=188-323 nM or >10 uM)) (PubMed:16267209, PubMed:27129258, PubMed:28880874, PubMed:29703751). It acts by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current (PubMed:16267209). It shows moderate affinity for lipid bilayers (PubMed:29703751). On Nav1.7/SCN9A, it has been shown to interact with the S3-S4 loop of domain DII (site 4) (PubMed:27129258). Is significantly more potent against Nav1.2/SCN2A than the other Nav channel subtypes (PubMed:16267209). In vivo, this toxin causes general ataxia, lack of response to stimuli, and semiparalysis (PubMed:16267209). After a few minutes, the mice are unable to stand, and breathing is reduced in rhythm and intensity (PubMed:16267209). Symptoms gradually increase with progressive slowing of breathing and flaccid paralysis, death occurred within 10 to 20 minutes post injection (PubMed:16267209). Animals remain totally flaccid, and no symptoms of excitatory neurotoxicity are observed (PubMed:16267209). {ECO:0000269|PubMed:16267209, ECO:0000269|PubMed:27129258, ECO:0000269|PubMed:28880874, ECO:0000269|PubMed:29703751}.

Target

voltage-gated sodium channel (Nav) voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Ceratogyrus marshalli (Straighthorned baboon tarantula) (Ceratogyrus cornuatus)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Inhibits many voltage-gated sodium channels and one voltage-gated calcium channel (Cav2.2/CACNA1B (IC(50)=400 nM), Nav1.2/SCN2A (IC(50)=3-70 nM), Nav1.1/SCN1A (IC(50)=523-1060 nM), Nav1.7/SCN9A (IC(50)=129.1-5120 nM), Nav1.4/SCN4A (IC(50)=263-888 nM or >10 uM) and Nav1.5/SCN5A (IC(50)=188-323 nM or >10 uM)) (PubMed:16267209, PubMed:27129258, PubMed:28880874, PubMed:29703751). It acts by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current (PubMed:16267209).
Beta-theraphotoxin-Cm1a (Beta-TRTX-Cm1a) (CcoTx-I) (Ceratotoxin-1) (CcoTx1)
spider
Neurotoxin 10 (Hwtx-1) family, 04 (CcoTx1) subfamily
Evidence at protein level
3D-structure Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16267209}.
Additional Detail Fields 7 fields
3D-structure Amidation Calcium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Pharmaceutical Secreted Toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 10 (Hwtx-1) family, 04 (CcoTx1) subfamily
Beta-theraphotoxin-Cm1a (Beta-TRTX-Cm1a) (CcoTx-I) (Ceratotoxin-1) (CcoTx1)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:16267209}.
spider