TPDB

3D PDB MODEL

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TPDB ID TPDB27741

Activity

Neurotoxin Toxicity

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2 Peptipedia pep-lab ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 29-aa standard natural multi-activity (Neurotoxin and Toxicity) peptide sequence curated from NTxPred2, Peptipedia, pep-lab, and other sources, with an available 3D structural model.

Sequence

GCKLTFWKCKNKKECCGWNACALGICMPR

FASTA

>TPDB27741|Neurotoxin;Toxicity|length=29
GCKLTFWKCKNKKECCGWNACALGICMPR

Formula C142H227N41O35S7

Absent Amino Acids DQHSYV

Common Amino Acids C

Mass 3293.04

PI 8.90

Basic Residues 6

Acidic Residues 1

Hydrophobic Residues 9

Net Charge +5

Boman Index 0.97

Hydrophobicity -0.152

Aliphatic Index 47.24

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 11375

Absorbance 280nm 345.43

Polar Residues 9

number

2

A

1

R

2

N

0

D

6

C

1

E

0

Q

3

G

0

H

1

I

2

L

5

K

1

M

1

F

1

P

0

S

1

T

2

W

0

Y

0

V

Amino Acid Distribution

A: 2 R: 1 N: 2 D: 0 C: 6 E: 1 Q: 0 G: 3 H: 0 I: 1 L: 2 K: 5 M: 1 F: 1 P: 1 S: 0 T: 1 W: 2 Y: 0 V: 0

Length

29

Formula

C142H227N41O35S7

Mass

3293.04

PI

8.90

Net Charge

+5

Boman Index

0.97

Hydrophobicity

-0.152

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Insect and vertebrate active toxin. Binds at site 4 of mammalian voltage-gated sodium channels and shifts the activation voltage of the mammalian rNav1.2a (SCN2A) channel to more hyperpolarized voltages, whereas the insect channel, DmNav1 (para), is not affected. Causes temporary paralysis when injected into lepidopteran larvae at 8.6 nmol/g. A low intracranial injection dose into mice causes lacrimation, closure of the eyes and sweating. A high injection dose causes extensive lacrimation and death.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Macrothele raveni (Funnel-web spider)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Insect and vertebrate active toxin. Binds at site 4 of mammalian voltage-gated sodium channels and shifts the activation voltage of the mammalian rNav1.2a (SCN2A) channel to more hyperpolarized voltages, whereas the insect channel, DmNav1 (para), is not affected.

Protein Name Beta-hexatoxin-Mr1a (Beta-HXTX-Mr1a) (Raventoxin III) (Raventoxin-3)

Venom Taxon Group spider

Protein Family Neurotoxin 15 family, 01 (magi-5) subfamily

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland.

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 15 family, 01 (magi-5) subfamily

Protein_Name

Beta-hexatoxin-Mr1a (Beta-HXTX-Mr1a) (Raventoxin III) (Raventoxin-3)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland.

Venom_Taxon_Group

spider