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Peptide record

TPDB26286

C24-LMWP LMWP LMWP-TAZ lowmolecular weight protamine (LMWP) Protamine Antibacterial Antifungal Antiparasitic Blood-Brain Barrier Cell-penetrating Peptides Hemolysis Toxicity standard
14 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB26286
C24-LMWP LMWP LMWP-TAZ lowmolecular weight protamine (LMWP) Protamine
Antibacterial Antifungal Antiparasitic Blood-Brain Barrier Cell-penetrating Peptides Hemolysis Toxicity
Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
Peptipedia Antifungipept CAFPdb dbAMP dbAMP 3.0 i2APP B3Pred BBPpredict CellPPD-MOD CPPsite3.0 PerseuCPP HemoPI Hemolytic2 PD_Hemolysis
standard
No
A 14-aa standard natural multi-activity (Antibacterial, Antifungal, Antiparasitic, and other sources) peptide sequence curated from Peptipedia, Antifungipept, CAFPdb, and other sources, with an available 3D structural model.
Sequence
VSRRRRRRGGRRRR
Physicochemical Analysis
C72H142N44O16
ANDCEQHILKMFPTWY
R
1880.20
13.35
10
0
1
+10
8.32
-3.029
20.71
Mammalian: 100 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
1
Residue Composition
number
0
A
10
R
0
N
0
D
0
C
0
E
0
Q
2
G
0
H
0
I
0
L
0
K
0
M
0
F
0
P
1
S
0
T
0
W
0
Y
1
V
Amino Acid Distribution
A: 0 R: 10 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 1 T: 0 W: 0 Y: 0 V: 1
Chemical Descriptors
14
C72H142N44O16
1880.20
13.35
+10
8.32
-3.029
Free
L
Linear
Cationic
Linear
Free
Similarity
Cationic
ValSerArgArgArgArgArgArgGlyGlyArgArgArgArg
N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)O NCC(=O)NCC(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)O
Evidence Records 16 records
Evidence 1 Activity

Activity

Blood-Brain Barrier

Assay & Model

Origin/Source
10 microliter per nostril
In vivo imaging
Peptide + BSA combination deleiveerd in brain olfactory bulb and littlebit in cerebral

Source & Reference

NA
B3Pdb

Other

Derived from protamine
Intranasal
peptide can deliver drug into brain tissue
Peptide+BSA
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Pluripotent mouse mesenchymal progenitor cell line (C3H10T1/2)

Assay & Model

New Zealand White male rabbits
New Zealand White male rabbits

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Synthetic
Protein [PDZ-binding motif (TAZ)]
Cytoplasm and nucleus
Higher uptake efficency
In vitro and in vivo
CCSSSSSSGGGSCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

A549/T and MCF-7/ADR

Assay & Model

BALB/c-nu nude mice
BALB/c-nu nude mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Nanoparticle (LMWP/PLGA nanoparticles), Small molecule drug (Doxorubicin)
Nucleus
Higher uptake efficiency
Endocytic pathway
In vitro and in vivo
CCSSSSSSGGGSCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Cultured Human Dental Pulp Stem Cells (hDPSCs)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
Protein [TAZ (transcriptional co-activator with PDZ-binding motif)]
Cytoplasm and nucleus
High
In vitro
CCSSSSSSGGGSCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

hMSC

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
Nucleic acid (miRNA)
In vitro
CCSSSSSSGGGSCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

MDA-MB-231 And MDA-MB-231-Egfp Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
FITC-labeled anti-EGFP siRNA
Cytoplasm and nucleus
Cellular uptake of the LMWP-PEG-S-S-siRNA conjugates was 50.6-fold higher than native siRNA without LMWP modification.
In vitro
CCSSSSSSGGGSCC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Target

Rm-1 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
GFP mRNA and PP7 VLPs
Cytoplasm
Higher level of GFP mRNA was observed in cells incubated with 2PP7-Protamine-GFP VLPs
In vitro
CCSSSSSSGGGSCC
Evidence 8 Activity

Activity

Cell-penetrating Peptides

Target

MDA-MB-231 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
FER siRNA
In vitro
CCCCCCSCCSSSSCC
Evidence 9 Activity

Activity

Cell-penetrating Peptides

Target

Hek293T And Ct26.Cl25 Cell Lines

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
5-methoxyuridine-modified mCherry mRNA, Enhanced green fluorescence protein (EGFP) mRNA and Ovalbumin (OVA) mRNA
Cytoplasm
Exhibited poor uptake efficiency with 24.1% for LMWP
In vitro
CCCCCCCCCCCC
Evidence 10 Activity

Activity

Cell-penetrating Peptides

Target

A549 And A549T Cells.

Assay & Model

Four-week-old female BALB/c nude mice
Four-week-old female BALB/c nude mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Paclitaxel (PTX)-Succinic acid (SA)
The uptake of PTX-LMWP was stronger than that of PTX-TAT in A549 cells at all incubation times (p<0.05),
Energy-independent direct translocation
In vitro and in vivo
CCSTTTTGGGTCCC
Evidence 11 Activity

Activity

Cell-penetrating Peptides

Assay & Model

BALB/c mice
BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
CY5/FITC labelled-Three proteins, bovine serum albumin (BSA), peroxidase (HRP) and β-galactosidase (β-gal)
Endocytosis
In vivo
CCSSTTTTSSCC
Evidence 12 Activity

Activity

Cell-penetrating Peptides

Target

HEI-OC1 cells

Assay & Model

Guinea pigs (250–300 g)
Guinea pigs (250–300 g)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
coumarin-6-loaded PLGA-NPs and P407-PLGA-NPs
LMWP group had stronger fluorescence, followed by penetratin, TAT and R8 groups
In vitro and in vivo
CTTTTTTTHHHHHHHHHHHHHHHTHHHHHHHSHHHHHHHHTTTTHHHHHHHHHHHHHHHHTTTGGGCC
Evidence 13 Activity

Activity

50% Hemolysis
Toxicity
69.89±0.08 µg/mL
Hemolytic Cytotoxic
50% Hemolysis | 69.89±0.08 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Leishmanicidal activity of synthetic antimicrobial peptides in an infection model with human dendritic cells. | Peptides | 2011
Evidence 14 Activity

Activity

50% Cell death
Toxicity
22.28±0.37 µg/mL
Cytotoxic
50% Cell death | 22.28±0.37 | µg/mL | target cell: Human dendritic cells

Target

Human dendritic cells

Source & Reference

NA
DBAASP
Leishmanicidal activity of synthetic antimicrobial peptides in an infection model with human dendritic cells. | Peptides | 2011
Evidence 15 Activity

Activity

Toxicity
Hemolytic Cytotoxic
Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

erythrocytes

Source & Reference

NA
DRAMP
J Control Release. 2014 Oct 28 192:47-56.
Evidence 16 Hemolysis

Source & Reference

HemoPI PD_Hemolysis
PD_Hemolysis
Additional Detail Fields 2 fields
CCSSSSSSGGGSCC CCCCCCSCCSSSSCC CCCCCCCCCCCC CCSTTTTGGGTCCC CCSSTTTTSSCC CTTTTTTTHHHHHHHHHHHHHHHTHHHHHHHSHHHHHHHHTTTTHHHHHHHHHHHHHHHHTTTGGGCC
Intranasal