TPDB

3D PDB MODEL

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TPDB ID TPDB25836

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 30-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

SWLSKSVKKLVNKKNYTRLEKLAKKKLFNE

FASTA

>TPDB25836|Antibacterial;Toxicity|length=30
SWLSKSVKKLVNKKNYTRLEKLAKKKLFNE

Formula C167H282N46O43

Absent Amino Acids DCQGHIMP

Common Amino Acids K

Mass 3622.36

PI 10.84

Basic Residues 10

Acidic Residues 2

Hydrophobic Residues 11

Net Charge +8

Boman Index 8.80

Hydrophobicity -1.013

Aliphatic Index 87.67

Half Life

Mammalian: 1.9 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 6990

Absorbance 280nm 192.97

Polar Residues 8

number

1

A

1

R

3

N

0

D

0

C

2

E

0

Q

0

G

0

H

0

I

5

L

9

K

0

M

1

F

0

P

3

S

1

T

1

W

1

Y

2

V

Amino Acid Distribution

A: 1 R: 1 N: 3 D: 0 C: 0 E: 2 Q: 0 G: 0 H: 0 I: 0 L: 5 K: 9 M: 0 F: 1 P: 0 S: 3 T: 1 W: 1 Y: 1 V: 2

Length

30

Formula

C167H282N46O43

Mass

3622.36

PI

10.84

Net Charge

+8

Boman Index

8.80

Hydrophobicity

-1.013

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 1 ug/ml), S. enteritidis (MIC 2-4 ug/ml), and S.aureus ATCC 29213 (MIC >128 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 2-4 µg/mL

Target

Target Organism S. enteritidis

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 1 ug/ml), S. enteritidis (MIC 2-4 ug/ml), and S.aureus ATCC 29213 (MIC >128 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >128 µg/mL

Target

Target Organism S.aureus ATCC 29213

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 1 ug/ml), S. enteritidis (MIC 2-4 ug/ml), and S.aureus ATCC 29213 (MIC >128 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µg/mL | target cell: Pig erythrocytes

Target

Target Cell Pig erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Evidence 5 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Cytotoxic

Toxicity Raw

NA | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Associating Biological Activity and Predicted Structure of Antimicrobial Peptides from Amphibians and Insects. | Antibiotics (Basel) | 2022