TPDB

3D PDB MODEL

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TPDB ID TPDB25060

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 24-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

RRFFFATAPCGYSRKFCKITRRKR

FASTA

>TPDB25060|Antibacterial;Toxicity|length=24
RRFFFATAPCGYSRKFCKITRRKR

Formula C135H215N45O29S2

Absent Amino Acids NDEQHLMWV

Common Amino Acids R

Mass 2996.60

PI 12.13

Basic Residues 9

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +9

Boman Index 2.11

Hydrophobicity -0.829

Aliphatic Index 24.58

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 1615

Absorbance 280nm 53.89

Polar Residues 6

number

2

A

6

R

0

N

0

D

2

C

0

E

0

Q

1

G

0

H

1

I

0

L

3

K

0

M

4

F

1

P

1

S

2

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 2 R: 6 N: 0 D: 0 C: 2 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 0 K: 3 M: 0 F: 4 P: 1 S: 1 T: 2 W: 0 Y: 1 V: 0

Length

24

Formula

C135H215N45O29S2

Mass

2996.60

PI

12.13

Net Charge

+9

Boman Index

2.11

Hydrophobicity

-0.829

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 16-32 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 16-32 ug/ml), S. enteritidis (MIC 32-64 ug/ml), and S.aureus ATCC 29213 (MIC >128 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 32-64 µg/mL

Target

Target Organism S. enteritidis

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 16-32 ug/ml), S. enteritidis (MIC 32-64 ug/ml), and S.aureus ATCC 29213 (MIC >128 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >128 µg/mL

Target

Target Organism S.aureus ATCC 29213

Source & Reference

Database Source NA

Other

Evidence Text

ctive against E. coli ATCC 25922 (MIC 16-32 ug/ml), S. enteritidis (MIC 32-64 ug/ml), and S.aureus ATCC 29213 (MIC >128 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µg/mL | target cell: Pig erythrocytes

Target

Target Cell Pig erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Associating Biological Activity and Predicted Structure of Antimicrobial Peptides from Amphibians and Insects | Antibiotics (Basel) | 2022