TPDB

3D PDB MODEL

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TPDB ID TPDB22559

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia CancerPPD 2.0 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 18-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from Peptipedia, CancerPPD 2.0, and PD_Hemolysis, with an available 3D structural model.

Sequence

KWCFRVCYSGICYSRCSG

FASTA

>TPDB22559|Antibacterial;Anticancer;Hemolysis;Toxicity|length=18
KWCFRVCYSGICYSRCSG

Formula C92H136N26O24S4

Absent Amino Acids ANDEQHLMPT

Common Amino Acids C

Mass 2118.49

PI 8.70

Basic Residues 3

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +3

Boman Index -3.04

Hydrophobicity 0.106

Aliphatic Index 37.78

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 8730

Absorbance 280nm 412.09

Polar Residues 9

number

0

A

2

R

0

N

0

D

4

C

0

E

0

Q

2

G

0

H

1

I

0

L

1

K

0

M

1

F

0

P

3

S

0

T

1

W

2

Y

1

V

Amino Acid Distribution

A: 0 R: 2 N: 0 D: 0 C: 4 E: 0 Q: 0 G: 2 H: 0 I: 1 L: 0 K: 1 M: 0 F: 1 P: 0 S: 3 T: 0 W: 1 Y: 2 V: 1

Length

18

Formula

C92H136N26O24S4

Mass

2118.49

PI

8.70

Net Charge

+3

Boman Index

-3.04

Hydrophobicity

0.106

Evidence 1 Activity

Activity

Detail Source Activity Hemolysis

Hemolysis Raw

Human RBCs: CC50=15.3±1.4 µM

Target

RBC Target Human RBCs

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

ACS Chem Biol. 2019 Dec 20 14(12):2895-2908.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 15.3±1.4 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 15.3±1.4 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties. | ACS Chem Biol | 2019

Evidence 3 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human RBCs: CC50=15.3±1.4 µM | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

ACS Chem Biol. 2019 Dec 20 14(12):2895-2908.

Evidence 4 Hemolysis

Activity

Hemolysis Raw

Human RBCs: CC50=15.3±1.4 µM

Detail Source Activity Hemolysis

Target

RBC Target Human RBCs

Source & Reference

source PD_Hemolysis

Reference

ACS Chem Biol. 2019 Dec 20 14(12):2895-2908.

Source DRAMP4

Database Source PD_Hemolysis