TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB21963

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia CAFPdb dbAMP PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 4-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from Peptipedia, CAFPdb, dbAMP, and other sources, with an available 3D structural model.

Sequence

KKRK

FASTA

>TPDB21963|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=4
KKRK

Formula C24H50N10O5

Absent Amino Acids ANDCEQGHILMFPSTWYV

Common Amino Acids K

Mass 558.73

PI 11.73

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +4

Boman Index 3.78

Hydrophobicity -4.050

Aliphatic Index 0.00

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

0

A

1

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

3

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 3 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

4

Formula

C24H50N10O5

Mass

558.73

PI

11.73

Net Charge

+4

Boman Index

3.78

Hydrophobicity

-4.050

Evidence 1 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Hemolysis

Activity Measurement 96.7

Hemolysis Raw

Human erythrocytes:HC50=96.7±1.4µg/ml

Target

RBC Target Human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Int J Mol Sci. 2020 Sep 29 21(19):7208.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 96.7±1.4 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 96.7±1.4 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effect of Disulfide Cyclization of Ultrashort Cationic Lipopeptides on Antimicrobial Activity and Cytotoxicity. | Int J Mol Sci | 2020

Evidence 3 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 2.4±0.4 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 2.4±0.4 | µg/mL | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effect of Disulfide Cyclization of Ultrashort Cationic Lipopeptides on Antimicrobial Activity and Cytotoxicity. | Int J Mol Sci | 2020

Evidence 4 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 96.7

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Human erythrocytes:HC50=96.7±1.4µg/ml | Cytotoxicity: HaCat: IC50=2.4±0.4µg/ml

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Int J Mol Sci. 2020 Sep 29 21(19):7208.

Evidence 5 Hemolysis

Activity

Activity Measurement 96.7

Activity Endpoint HC50

Hemolysis Raw

Human erythrocytes:HC50=96.7±1.4µg/ml

Detail Source Activity Hemolysis

Target

RBC Target Human erythrocytes

Source & Reference

source PD_Hemolysis

Reference

Int J Mol Sci. 2020 Sep 29 21(19):7208.

Source DRAMP4

Database Source PD_Hemolysis