Back to search

Peptide record

TPDB21868

Tripeptide 3 K3 Antibacterial Anticancer Antifungal Cell-penetrating Peptides Hemolysis Toxicity Selfassembly standard
3 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB21868
Tripeptide 3 K3
Antibacterial Anticancer Antifungal Cell-penetrating Peptides Hemolysis Toxicity Selfassembly
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides Self-assembling and biomaterial peptides
Peptipedia CancerPPD 2.0 CAFPdb dbAMP CPPsite3.0 PD_Hemolysis SAPdb
standard
No
A 3-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from Peptipedia, CancerPPD 2.0, CAFPdb, and other sources, with an available 3D structural model.
Sequence
KKK
Physicochemical Analysis
C18H38N6O4
ARNDCEQGHILMFPSTWYV
K
402.54
10.84
3
0
0
+3
2.97
-3.900
0.00
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
0
0.00
0
Residue Composition
number
0
A
0
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
0
I
0
L
3
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 3 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
3
C18H38N6O4
402.54
10.84
+3
2.97
-3.900
Free
L
Linear
Free
Self-Assembly Information 1 records
Record 1 Selfassembly

Source

22543454
10.1016/j.compbiomed.2021.104391
SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly

Self-Assembly

Nanostructure formation
Nanosphere
solvent: Water concentration: 200 μM
Selfassembly; Nanostructure=Nanosphere; Trigger=solvent: Water; concentration: 200 μM
Transmission Electron Microscopy (TEM), AFM (Atomic Force Microscopy)
Selfassembly
Evidence Records 16 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

MCF7 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Non-natural residues Linear L
Synthetic
HF488 labelled PNAs with 2-aminopyridine (M)
Cytoplasm and nucleoli
Less uptake than Tat and octa-arginine conjugate
In vitro
Evidence 2 Activity

Activity

HC50
Hemolysis
90.0
Human erythrocytes:HC50=90.0 ± 6.5 µg/ml

Target

Human erythrocytes

Source & Reference

Hemolytik PD_Hemolysis
DRAMP4
Int J Mol Sci. 2020 Sep 29 21(19):7208.

Other

Source Activity Label Source Definition
Hemolysis
Hemolytik experimentally validated hemolytic peptide annotation.
Evidence 3 Activity

Activity

10% Hemolysis
Toxicity
512 µg/mL
Hemolytic Cytotoxic
10% Hemolysis | 512 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Cationic Net Charge and Counter Ion Type as Antimicrobial Activity Determinant Factors of Short Lipopeptides. | Front Microbiol | 2017
Evidence 4 Activity

Activity

10% Hemolysis
Toxicity
>4000 µg/mL
Hemolytic Cytotoxic
10% Hemolysis | >4000 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Cationic Net Charge and Counter Ion Type as Antimicrobial Activity Determinant Factors of Short Lipopeptides. | Front Microbiol | 2017
Evidence 5 Activity

Activity

50% Hemolysis
Toxicity
60 µM
Hemolytic Cytotoxic
50% Hemolysis | 60 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Ultrashort peptide bioconjugates are exclusively antifungal agents and synergize with cyclodextrin and amphotericin B. | Antimicrob Agents Chemother | 2012
Evidence 6 Activity

Activity

1% Hemolysis
Toxicity
100 µg/mL
Hemolytic Cytotoxic
1% Hemolysis | 100 | µg/mL | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Investigating the antimicrobial peptide 'window of activity' using cationic lipopeptides with hydrocarbon and fluorinated tails. | Int J Antimicrob Agents | 2012
Evidence 7 Activity

Activity

10% Hemolysis
Toxicity
256 µg/mL
Hemolytic Cytotoxic
10% Hemolysis | 256 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Cationic Net Charge and Counter Ion Type as Antimicrobial Activity Determinant Factors of Short Lipopeptides. | Front Microbiol | 2017
Evidence 8 Activity

Activity

4% Hemolysis
Toxicity
100 µg/mL
Hemolytic Cytotoxic
4% Hemolysis | 100 | µg/mL | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Investigating the antimicrobial peptide 'window of activity' using cationic lipopeptides with hydrocarbon and fluorinated tails. | Int J Antimicrob Agents | 2012
Evidence 9 Activity

Activity

10% Hemolysis
Toxicity
32 µg/mL
Hemolytic Cytotoxic
10% Hemolysis | 32 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Cationic Net Charge and Counter Ion Type as Antimicrobial Activity Determinant Factors of Short Lipopeptides. | Front Microbiol | 2017
Evidence 10 Activity

Activity

50% Hemolysis
Toxicity
90±6.5 µg/mL
Hemolytic Cytotoxic
50% Hemolysis | 90±6.5 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Effect of Disulfide Cyclization of Ultrashort Cationic Lipopeptides on Antimicrobial Activity and Cytotoxicity. | Int J Mol Sci | 2020
Evidence 11 Activity

Activity

IC50
Toxicity
5.2±0.6 µg/mL
Cytotoxic
IC50 | 5.2±0.6 | µg/mL | target cell: Human keratinocytes HaCat

Target

Human keratinocytes HaCat

Source & Reference

NA
DBAASP
Effect of Disulfide Cyclization of Ultrashort Cationic Lipopeptides on Antimicrobial Activity and Cytotoxicity. | Int J Mol Sci | 2020
Evidence 12 Activity

Activity

62% Hemolysis
Toxicity
100 µg/mL
Hemolytic Cytotoxic
62% Hemolysis | 100 | µg/mL | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Investigating the antimicrobial peptide 'window of activity' using cationic lipopeptides with hydrocarbon and fluorinated tails. | Int J Antimicrob Agents | 2012
Evidence 13 Activity

Activity

2% Hemolysis
Toxicity
100 µg/mL
Hemolytic Cytotoxic
2% Hemolysis | 100 | µg/mL | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
Investigating the antimicrobial peptide 'window of activity' using cationic lipopeptides with hydrocarbon and fluorinated tails. | Int J Antimicrob Agents | 2012
Evidence 14 Activity

Activity

HC50
Toxicity
90.0
Hemolytic Cytotoxic
Hemolytic_activity: Human erythrocytes:HC50=90.0 ± 6.5 µg/ml | Cytotoxicity: HaCat: IC50=5.2 ± 0.6 µg/ml

Target

erythrocytes

Source & Reference

NA
DRAMP
Int J Mol Sci. 2020 Sep 29 21(19):7208.
Evidence 15 Hemolysis

Activity

90.0
HC50
Human erythrocytes:HC50=90.0 ± 6.5 µg/ml
Hemolysis

Target

Human erythrocytes

Source & Reference

PD_Hemolysis
Int J Mol Sci. 2020 Sep 29 21(19):7208.
DRAMP4
PD_Hemolysis
Evidence 16 Selfassembly

Activity

Nanostructure formation
Selfassembly Nanostructure=Nanosphere Trigger=solvent: Water concentration: 200 μM
Nanosphere

Source & Reference

SAPdb
10.1016/j.compbiomed.2021.104391

Other

None specified
solvent: Water concentration: 200 μM
Transmission Electron Microscopy (TEM), AFM (Atomic Force Microscopy)
22543454
SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly
SAPdb ID NA low-detail seed row from experimentally curated self-assembly database.
Additional Detail Fields 5 fields
solvent: Water concentration: 200 μM
None specified
SAPdb ID NA low-detail seed row from experimentally curated self-assembly database.
22543454
SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly