TPDB

3D PDB MODEL

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TPDB ID TPDB21836

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 23-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

KIKIPWGKVKDFLVGGMKAVGKK

FASTA

>TPDB21836|Antibacterial;Toxicity|length=23
KIKIPWGKVKDFLVGGMKAVGKK

Formula C120H203N31O26S1

Absent Amino Acids RNCEQHSTY

Common Amino Acids K

Mass 2528.18

PI 10.92

Basic Residues 7

Acidic Residues 1

Hydrophobic Residues 10

Net Charge +6

Boman Index 4.30

Hydrophobicity -0.130

Aliphatic Index 93.04

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 217.55

Polar Residues 0

number

1

A

0

R

0

N

1

D

0

C

0

E

0

Q

4

G

0

H

2

I

1

L

7

K

1

M

1

F

1

P

0

S

0

T

1

W

0

Y

3

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 1 C: 0 E: 0 Q: 0 G: 4 H: 0 I: 2 L: 1 K: 7 M: 1 F: 1 P: 1 S: 0 T: 0 W: 1 Y: 0 V: 3

Length

23

Formula

C120H203N31O26S1

Mass

2528.18

PI

10.92

Net Charge

+6

Boman Index

4.30

Hydrophobicity

-0.130

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1-2 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 1-2 ug/ml), S. enteritidis (MIC 4-8 ug/ml), and S. aureus ATCC 29213 (MIC 4-8 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4-8 µg/mL

Target

Target Organism S. enteritidis

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 1-2 ug/ml), S. enteritidis (MIC 4-8 ug/ml), and S. aureus ATCC 29213 (MIC 4-8 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4-8 µg/mL

Target

Target Organism Staphylococcus aureus ATCC 29213

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 1-2 ug/ml), S. enteritidis (MIC 4-8 ug/ml), and S. aureus ATCC 29213 (MIC 4-8 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µg/mL | target cell: Pig erythrocytes

Target

Target Cell Pig erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Evidence 5 Activity

Activity

Activity Endpoint 60% Cytotoxicity

Detail Source Activity Toxicity

Activity Measurement 128 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

60% Cytotoxicity | 128 | µg/mL | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Associating Biological Activity and Predicted Structure of Antimicrobial Peptides from Amphibians and Insects. | Antibiotics (Basel) | 2022