TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB21685

Peptide_Name

Z4

Activity

Antibacterial Anticancer Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural multi-activity (Antibacterial, Anticancer, and Toxicity) peptide sequence curated from Peptipedia, AntiCP 2.0, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

KAAKKAWKAAKKAAKWWKKAA

FASTA

>TPDB21685|Antibacterial;Anticancer;Toxicity|length=21
KAAKKAWKAAKKAAKWWKKAA

Formula C114H185N33O22

Absent Amino Acids RNDCEQGHILMFPSTYV

Common Amino Acids AK

Mass 2369.93

PI 11.41

Basic Residues 9

Acidic Residues 0

Hydrophobic Residues 12

Net Charge +9

Boman Index 1.83

Hydrophobicity -1.029

Aliphatic Index 42.86

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 16500

Absorbance 280nm 696.22

Polar Residues 0

number

9

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

9

K

0

M

0

F

0

P

0

S

0

T

3

W

0

Y

0

V

Amino Acid Distribution

A: 9 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 9 M: 0 F: 0 P: 0 S: 0 T: 0 W: 3 Y: 0 V: 0

Length

21

Formula

C114H185N33O22

Mass

2369.93

PI

11.41

Net Charge

+9

Boman Index

1.83

Hydrophobicity

-1.029

C_terminal_Modification

Free

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Anticancer

Origin

Synthetic

Evidence 1 Activity

Activity

Activity Endpoint EC50

Activity Raw

EC50 =155 µM

Detail Source Activity Anticancer

Activity Measurement 155 µM

Target

Cancer Type Skin Cancer

Cell Line Meth A

Tissue Affected Skin

Assay & Model

Assay MTT/MTS assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2011

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint EC50

Activity Raw

EC50 =68 µM

Detail Source Activity Anticancer

Activity Measurement 68 µM

Target

Cancer Type Breast Cancer

Cell Line MT-1

Tissue Affected Breast

Assay & Model

Assay MTT/MTS assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2011

Evidence 3 Activity

Activity

Activity Endpoint EC50

Activity Raw

EC50 =173 µM

Detail Source Activity Anticancer

Activity Measurement 173 µM

Target

Cancer Type Colon Cancer

Cell Line HT-29

Tissue Affected Colon

Assay & Model

Assay MTT/MTS assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2011

Evidence 4 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Toxicity

Activity Measurement 78 µM

Toxicity Type Cytotoxic

Toxicity Raw

EC50 | 78 | µM | target cell: Human fibroblasts MRC-5

Target

Target Cell Human fibroblasts MRC-5

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Relative spatial positions of tryptophan and cationic residues in helical membrane-active peptides determine their cytotoxicity. | J Biol Chem | 2012

Evidence 5 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: MRC-5: EC53=78 µM | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Biol Chem. 2012 Jan 2 287(1):233-244.