TPDB

3D PDB MODEL

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TPDB ID TPDB20823

Activity

Antibacterial Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 19-aa standard natural multi-activity (Antibacterial and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

GWLDIAKKVASVVAGLGKR

FASTA

>TPDB20823|Antibacterial;Toxicity|length=19
GWLDIAKKVASVVAGLGKR

Formula C90H154N26O23

Absent Amino Acids NCEQHMFPTY

Common Amino Acids AGKV

Mass 1968.37

PI 10.83

Basic Residues 4

Acidic Residues 1

Hydrophobic Residues 10

Net Charge +3

Boman Index 1.59

Hydrophobicity 0.395

Aliphatic Index 123.16

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 279.42

Polar Residues 1

number

3

A

1

R

0

N

1

D

0

C

0

E

0

Q

3

G

0

H

1

I

2

L

3

K

0

M

0

F

0

P

1

S

0

T

1

W

0

Y

3

V

Amino Acid Distribution

A: 3 R: 1 N: 0 D: 1 C: 0 E: 0 Q: 0 G: 3 H: 0 I: 1 L: 2 K: 3 M: 0 F: 0 P: 0 S: 1 T: 0 W: 1 Y: 0 V: 3

Length

19

Formula

C90H154N26O23

Mass

1968.37

PI

10.83

Net Charge

+3

Boman Index

1.59

Hydrophobicity

0.395

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4-8 µg/mL

Target

Target Organism Escherichia coli ATCC 25922

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 4-8 ug/ml), S. enteritidis (MIC 64-128 ug/ml), and S. aureus ATCC 29213 (MIC 64 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64-128 µg/mL

Target

Target Organism S. enteritidis

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 4-8 ug/ml), S. enteritidis (MIC 64-128 ug/ml), and S. aureus ATCC 29213 (MIC 64 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64 µg/mL

Target

Target Organism Staphylococcus aureus ATCC 29213

Source & Reference

Database Source NA

Other

Evidence Text

Active against E. coli ATCC 25922 (MIC 4-8 ug/ml), S. enteritidis (MIC 64-128 ug/ml), and S. aureus ATCC 29213 (MIC 64 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >128 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >128 | µg/mL | target cell: Pig erythrocytes

Target

Target Cell Pig erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Associating Biological Activity and Predicted Structure of Antimicrobial Peptides from Amphibians and Insects | Antibiotics (Basel) | 2022