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Peptide record

TPDB20800

Antibacterial Anticancer Toxicity standard

25 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB20800

Activity

Antibacterial Anticancer Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 25-aa standard natural multi-activity (Antibacterial, Anticancer, and Toxicity) peptide sequence curated from Peptipedia, with an available 3D structural model.

Sequence

GWGSFFKKAAHAGKHAGKAALTHYL

FASTA

>TPDB20800|Antibacterial;Anticancer;Toxicity|length=25
GWGSFFKKAAHAGKHAGKAALTHYL

Formula C125H184N36O29

Absent Amino Acids RNDCEQIMPV

Common Amino Acids A

Mass 2655.06

PI 10.64

Basic Residues 7

Acidic Residues 0

Hydrophobic Residues 12

Net Charge +4

Boman Index -0.04

Hydrophobicity -0.260

Aliphatic Index 55.20

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 6990

Absorbance 280nm 263.27

Polar Residues 3

number

Amino Acid Distribution

A: 6 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 3 I: 0 L: 2 K: 4 M: 0 F: 2 P: 0 S: 1 T: 1 W: 1 Y: 1 V: 0

Length

Formula

C125H184N36O29

Mass

2655.06

10.64

Net Charge

Boman Index

-0.04

Hydrophobicity

-0.260

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4-8 µg/mL

Target

Target Organism Klebsiella pneumoniae NCTC 13368 or M6

Source & Reference

Database Source NA

Other

Evidence Text

Active against Gram- K. pneumoniae NCTC 13368 or M6 (MIC 4-8 ug/ml), A. baumannii AYE or ATCC 17978 (MIC 1-2 ug/ml), P. aeruginosa NCTC 13437 or PAO1 (MIC 16

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1-2 µg/mL

Target

Target Organism A. baumannii AYE or ATCC 17978

Source & Reference

Database Source NA

Other

Evidence Text

Active against Gram- K. pneumoniae NCTC 13368 or M6 (MIC 4-8 ug/ml), A. baumannii AYE or ATCC 17978 (MIC 1-2 ug/ml), P. aeruginosa NCTC 13437 or PAO1 (MIC 16-32 ug/ml), E. coli NCTC 12923 (MIC 1 ug/ml), Gr

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 16-32 µg/mL

Target

Target Organism Pseudomonas aeruginosa NCTC 13437 or PAO1

Source & Reference

Database Source NA

Other

Evidence Text

umoniae NCTC 13368 or M6 (MIC 4-8 ug/ml), A. baumannii AYE or ATCC 17978 (MIC 1-2 ug/ml), P. aeruginosa NCTC 13437 or PAO1 (MIC 16-32 ug/ml), E. coli NCTC 12923 (MIC 1 ug/ml), Gram+ methicillin-sensitive S. aureus ATCC 9144 (MIC 8

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1 µg/mL

Target

Target Organism Escherichia coli NCTC 12923

Source & Reference

Database Source NA

Other

Evidence Text

ii AYE or ATCC 17978 (MIC 1-2 ug/ml), P. aeruginosa NCTC 13437 or PAO1 (MIC 16-32 ug/ml), E. coli NCTC 12923 (MIC 1 ug/ml), Gram+ methicillin-sensitive S. aureus ATCC 9144 (MIC 8 ug/ml), S. aureus MRSA NCTC 13616

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 8 µg/mL

Target

Target Organism Staphylococcus aureus ATCC 9144

Source & Reference

Database Source NA

Other

Evidence Text

or PAO1 (MIC 16-32 ug/ml), E. coli NCTC 12923 (MIC 1 ug/ml), Gram+ methicillin-sensitive S. aureus ATCC 9144 (MIC 8 ug/ml), S. aureus MRSA NCTC 13616 or MRSA NCTC 13277 (MIC 64-128 ug/ml), vancomycin-sensitive an

Evidence 6 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 64-128 µg/mL

Target

Target Organism Staphylococcus aureus MRSA NCTC 13616 or MRSA NCTC 13277

Source & Reference

Database Source NA

Other

Evidence Text

NCTC 12923 (MIC 1 ug/ml), Gram+ methicillin-sensitive S. aureus ATCC 9144 (MIC 8 ug/ml), S. aureus MRSA NCTC 13616 or MRSA NCTC 13277 (MIC 64-128 ug/ml), vancomycin-sensitive and resistant E. faecalis NCTC 775 or NCTC 12204 (MIC 32-128 ug/ml)

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 32-128 µg/mL

Target

Target Organism Enterococcus faecalis NCTC 775 or NCTC 12204

Source & Reference

Database Source NA

Other

Evidence Text

MRSA NCTC 13616 or MRSA NCTC 13277 (MIC 64-128 ug/ml), vancomycin-sensitive and resistant E. faecalis NCTC 775 or NCTC 12204 (MIC 32-128 ug/ml) (anti-VRE).

Evidence 8 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement >400 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | >400 | µg/mL | target cell: Human embryonic kidney HEK293 cells

Target

Target Cell Human embryonic kidney HEK293 cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy. | Commun Biol | 2020

Evidence 9 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic

Toxicity Raw

NA | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy. | Commun Biol | 2020

Evidence 10 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 400 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: HEK293: IC50~400 µg/ml

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Commun Biol. 2020 Nov 27 3(1):699.