TPDB

3D PDB MODEL

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TPDB ID TPDB20398

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 29-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from Peptipedia, HemoPI, Hemolytic2, and other sources, with an available 3D structural model.

Sequence

GLPVCGETCFTGSCYTPGCSCNWPVCNRN

FASTA

>TPDB20398|Antibacterial;Anticancer;Hemolysis;Toxicity|length=29
GLPVCGETCFTGSCYTPGCSCNWPVCNRN

Formula C127H190N36O41S6

Absent Amino Acids ADQHIKM

Common Amino Acids C

Mass 3069.48

PI 5.90

Basic Residues 1

Acidic Residues 1

Hydrophobic Residues 6

Net Charge -0

Boman Index 0.38

Hydrophobicity -0.028

Aliphatic Index 33.45

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 7365

Absorbance 280nm 239.94

Polar Residues 15

number

0

A

1

R

3

N

0

D

6

C

1

E

0

Q

4

G

0

H

0

I

1

L

0

K

0

M

1

F

3

P

2

S

3

T

1

W

1

Y

2

V

Amino Acid Distribution

A: 0 R: 1 N: 3 D: 0 C: 6 E: 1 Q: 0 G: 4 H: 0 I: 0 L: 1 K: 0 M: 0 F: 1 P: 3 S: 2 T: 3 W: 1 Y: 1 V: 2

Length

29

Formula

C127H190N36O41S6

Mass

3069.48

PI

5.90

Net Charge

-0

Boman Index

0.38

Hydrophobicity

-0.028

Evidence 1 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Hemolysis

Activity Measurement 4.29 µM

Hemolysis Raw

HRBCs: HC50=4.29 µM

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Peptides. 2010 Aug 31(8):1434-40.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 4.29 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 4.29 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Isolation and characterization of cytotoxic cyclotides from Viola tricolor. | Peptides | 2010

Evidence 3 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 4.29 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: HRBCs: HC50=4.29 µM | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Peptides. 2010 Aug 31(8):1434-40.

Evidence 4 Hemolysis

Activity

Activity Measurement 4.29 µM

Activity Endpoint HC50

Hemolysis Raw

HRBCs: HC50=4.29 µM

Detail Source Activity Hemolysis

Source & Reference

source HemoPI PD_Hemolysis

Reference

Peptides. 2010 Aug 31(8):1434-40.

Source DRAMP4

Database Source PD_Hemolysis