TPDB

3D PDB MODEL

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TPDB ID TPDB20386

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 29-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from Peptipedia, HemoPI, Hemolytic2, and other sources, with an available 3D structural model.

Sequence

GLPICGETCVGGTCNTPGCFCTWPVCTRN

FASTA

>TPDB20386|Antibacterial;Anticancer;Hemolysis;Toxicity|length=29
GLPICGETCVGGTCNTPGCFCTWPVCTRN

Formula C124H193N35O39S6

Absent Amino Acids ADQHKMSY

Common Amino Acids C

Mass 2990.47

PI 5.90

Basic Residues 1

Acidic Residues 1

Hydrophobic Residues 6

Net Charge -0

Boman Index 0.62

Hydrophobicity 0.286

Aliphatic Index 46.90

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5875

Absorbance 280nm 196.46

Polar Residues 13

number

0

A

1

R

2

N

0

D

6

C

1

E

0

Q

5

G

0

H

1

I

1

L

0

K

0

M

1

F

3

P

0

S

5

T

1

W

0

Y

2

V

Amino Acid Distribution

A: 0 R: 1 N: 2 D: 0 C: 6 E: 1 Q: 0 G: 5 H: 0 I: 1 L: 1 K: 0 M: 0 F: 1 P: 3 S: 0 T: 5 W: 1 Y: 0 V: 2

Length

29

Formula

C124H193N35O39S6

Mass

2990.47

PI

5.90

Net Charge

-0

Boman Index

0.62

Hydrophobicity

0.286

Evidence 1 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Hemolysis

Activity Measurement 11.53 µM

Hemolysis Raw

HRBCs: HC50=11.53 µM

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Peptides. 2010 Aug 31(8):1434-40.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 11.53 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 11.53 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Isolation and characterization of cytotoxic cyclotides from Viola tricolor. | Peptides | 2010

Evidence 3 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 11.53 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: HRBCs: HC50=11.53 µM | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Peptides. 2010 Aug 31(8):1434-40.

Evidence 4 Hemolysis

Activity

Activity Measurement 11.53 µM

Activity Endpoint HC50

Hemolysis Raw

HRBCs: HC50=11.53 µM

Detail Source Activity Hemolysis

Source & Reference

source HemoPI PD_Hemolysis

Reference

Peptides. 2010 Aug 31(8):1434-40.

Source DRAMP4

Database Source PD_Hemolysis